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BOR - Papers in Press, published online ahead of print May 3, 2006.
Biol Reprod 2006, 10.1095/biolreprod.106.052514
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BIOLOGY OF REPRODUCTION 75, 226–230 (2006)
DOI: 10.1095/biolreprod.106.052514
© 2006 by the Society for the Study of Reproduction, Inc.

Research Article

Cloned Transgenic Swine Via In Vitro Production and Cryopreservation1

Rongfeng Li 3 4, Liangxue Lai 3 45 , David Wax 4, Yanhong Hao 4, Clifton N. Murphy 4, August Rieke 4, Melissa Samuel 45 , Mike L. Linville 6, Scott W. Korte 7, Rhobert W. Evans 9, James R. Turk 8, Jing X. Kang 10, William T. Witt 11, Yifan Dai 11, and Randall S. Prather 2 45 

Division of Animal Science,4 National Swine Resource and Research Center,5 Office of Animal Resources,6 Department of Veterinary Pathobiology,7 Department of Veterinary Biomedical Sciences,8 University of Missouri–Columbia, Columbia, Missouri 65211 Department of Epidemiology,9 GSPH, University of Pittsburgh, Pittsburgh, Pennsylvania 15261 Department of Medicine,10 Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114 Thomas Starzl Transplantation Institute,11 Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15261

ABSTRACT

Ithas been notoriously difficult to successfully cryopreserve swine embryos, a task that has been even more difficult for in vitro-produced embryos. The first reproducible method of cryopreserving in vivo-produced swine embryos was after centrifugation and removal of the lipids. Here we report the adaptation of a similar process that permits the cryopreservation of in vitro-produced somatic cell nuclear transfer (SCNT) swine embryos. These embryos develop to the blastocyst stage and survive cryopreservation. Transfer of 163 cryopreserved SCNT embryos to two surrogates produced 10 piglets. Application of this technique may permit national and international movement of cloned transgenic swine embryos, storage until a suitable surrogate is available, or the long-term frozen storage of valuable genetics.

assisted reproductive technology, early development

FOOTNOTES

1 Supported by the National Center for Research Resources (RR18877, R.S.P.) and other Institutes at the NIH via the University of Iowa (HL51670, R.S.P.) and directly to Pittsburgh (DK64207, Y.D.), an unrestricted gift to the Thomas E. Starzl Transplantation Institute from the Robert E. Eberly Program for Transplant Innovation (Y.D.), a Missouri Life Sciences Fellowship (S.K.), and Food for the 21st Century (R.S.P.).

2 Correspondence: Randall S. Prather, 920 East Campus Dr., E125E ASRC, University of Missouri, Columbia, MO 65211. FAX: 573 884 7827; pratherr{at}missouri.edu

3 These authors contributed equally to this work.






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Copyright © 2006 by the Society for the Study of Reproduction.