Biol Reprod Lalor Postdoctoral Fellowships -- Application Deadline January 15, 2009
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

BOR - Papers in Press, published online ahead of print June 28, 2006.
Biol Reprod 2006, 10.1095/biolreprod.106.053330
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
75/4/524    most recent
biolreprod.106.053330v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Popova, E.
Right arrow Articles by Krivokharchenko, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Popova, E.
Right arrow Articles by Krivokharchenko, A.
Agricola
Right arrow Articles by Popova, E.
Right arrow Articles by Krivokharchenko, A.
BIOLOGY OF REPRODUCTION 75, 524–530 (2006)
DOI: 10.1095/biolreprod.106.053330
© 2006 by the Society for the Study of Reproduction, Inc.

Research Article

Full-Term Development of Rat after Transfer of Nuclei from Two-Cell Stage Embryos1

Elena Popova , Michael Bader 2, and Alexander Krivokharchenko 

Max-Delbrück Center for Molecular Medicine (MDC), D-13092 Berlin-Buch, Germany

ABSTRACT

Cloning technology would allow targeted genetic alterations in the rat, a species which is yet unaccessible for such studies due to the lack of germline-competent embryonic stem cells. The present study was performed to examine the developmental ability of reconstructed rat embryos after transfer of nuclei from early preimplantation stages. We observed that single blastomeres from two-cell embryos and zygotes reconstructed by pronuclei exchange can develop in vitro until morula/blastocyst stage. When karyoplasts from blastomeres were used for the reconstruction of embryos, highest in vitro cleavage rates were obtained with nuclei in an early phase of the cell cycle transferred into enucleated preactivated oocytes or zygotes. However, further in vitro development of reconstructed embryos produced from blastomere nuclei was arrested at early cleavage stages under all conditions tested in this study. In contrast, immediate transfer to foster mothers of reconstructed embryos with nuclei from two-cell embryos at an early stage of the cell cycle in preactivated enucleated oocytes resulted in live newborn rats, with a general efficiency of 0.4%–2.2%. The genetic origin of the cloned offspring was verified by using donor nuclei from embryos of Black Hooded Wistar rats and transgenic rats carrying an ubiquitously expressed green fluorescent protein transgene. Thus, we report for the first time the production of live cloned rats using nuclei from two-cell embryos.

embryo, oocyte development

FOOTNOTES

1 Supported through EURATools (European Commission contract no. LSHG-CT-2005–019015).

2 Correspondence. FAX: 49 30 9406 2110; mbader{at}mdc-berlin.de






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2006 by the Society for the Study of Reproduction.