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Restoration of Spermatogenesis and Male Fertility by Transplantation of Dispersed Testicular Cells in the Chicken¹

BIOPHARM,³ Research Institute of Biopharmacy and Veterinary Drugs, a.s. 254 49 Jílové u Prahy, Czech Republic Institute of Molecular Genetics,⁴ Academy of Sciences of the Czech Republic, CZ-16683 Prague 6, Czech Republic Biotechnology and Germplasm Laboratory,⁵ Agricultural Research Service, US Department of Agriculture, Beltsville, Maryland 20705 Station de Recherches Avicoles,⁶ Institut National de la Recherche Agronomique, Centre de Tours, 37380 Nouzilly, France

ABSTRACT

Transplantation of male germ cells into sterilized recipients has been widely used in mammals for conventional breeding and transgenesis purposes. This study presents a workable approach for germ cell transplantation between male chickens. Testicular cells from adult and prepubertal donors were dispersed and transplanted by injection directly into the testes of recipient males sterilized by repeated gamma irradiation. We describe the repopulation of the recipient seminiferous epithelium up to the production of heterologous sperm in about 50% of transplanted males. In comparison to males transplanted with testicular cell preparations from adult donors, in which the first ejaculates with sperm were recovered about 5 wk after transfer, a substantial interval (about 10 wk) was necessary to obtain ejaculates after the transfer of testicular cells from prepubertal donors. However, in both cases, recipient males produced ejaculates capable of fertilizing ova and producing progeny expressing donor genes.

developmental biology, gametogenesis, implantation, spermatogenesis, testis

¹ Supported by grant 1P05ME722 to P.T. from the Grant Agency of the Ministry of Education, Youth, and Sports and grant 523/04/0569 to J.H. awarded by the Grant Agency of the Czech Republic.

² Correspondence: FAX: 33 2 4742 7778; brillard{at}tours.inra.fr

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