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This Article Full Text Full Text (PDF) All Versions of this Article: 76/1/43    most recent biolreprod.106.054999v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Honaramooz, A. Articles by Dobrinski, I. Search for Related Content PubMed PubMed Citation Articles by Honaramooz, A. Articles by Dobrinski, I. Agricola Articles by Honaramooz, A. Articles by Dobrinski, I.

Building a Testis: Formation of Functional Testis Tissue after Transplantation of Isolated Porcine (Sus scrofa) Testis Cells¹

Center for Animal Transgenesis and Germ Cell Research, Department of Clinical Sciences, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, Pennsylvania 19348

ABSTRACT

During mammalian development, morphogenesis of the testis requires the coordinated interplay of somatic cells to form seminiferous cords in which the primitive germ cells reside. These cords are the precursor of the functional male gonad and as such form the basis of male fertility. Cell migration during mammalian organogenesis and formation of complex tissues, such as the testis, are difficult to study in situ. Herein, we report extensive rearrangement of cells to regenerate complete functional testis tissue after implantation of isolated neonatal porcine testis cells under the skin of immunodeficient mice. Somatic cells and germ cells reorganized into structures that have remarkable morphologic and physiologic similarity to normal testis tissue, forming the endocrine and spermatogenic compartment of the testis. This unique in vivo system provides an accessible model for the study of testicular morphogenesis that could be especially useful in nonrodent species.

assisted reproductive technology, germ cells, morphogenesis, pig, spermatogenesis, testis

³Current address: Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada S7N 5B4.

¹Supported by National Research Initiative Competitive Grant 2003-35203-13486 from the United States Department of Agriculture Cooperative State Research, Education, and Extension Service and by grant 5 R01 RR17359-05 from the National Center for Research Resources (NCRR), a component of the National Institutes of Health (NIH). The contents are solely the responsibility of the authors and do not necessarily represent the official views of the NCRR or the NIH.

Correspondence: ² FAX: 610 925 8121; e-mail: dobrinsk{at}vet.upenn.edu

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