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Differential Expression of Glucose Transporters in Rabbit Placenta: Effect of Hypercholesterolemia in Dams¹

Laboratoire de Physiologie Materno-Ftale,³ and Centre de Recherche BioMed,⁴ Université du Québec à Montréal, Montréal, Québec, Canada H3C 3P8

ABSTRACT

Low birth weight is observed in rabbit offspring when maternal hypercholesterolemia is induced during gestation, but the related etiology is still unknown. Glucose is one of the most important substances during fetal development, and defect in glucose supply to fetus was related to pathophysiological mechanisms in intrauterine growth restriction. Thus, the aim of this work was to evaluate the impact of maternal hypercholesterolemia during rabbit gestation on the glucose metabolism and the routing of glucose transporters (SLC2 and SLC5 [previously known as GLUT and SGLT]) in placenta. In this study, maternal and offspring serum levels of glucose and insulin were evaluated for control and hypercholesterolemic groups, and the mRNA and protein expressions of placental SLCs were quantified by real-time RT-PCR and Western immunoblot, respectively. Our data demonstrate that maternal hypercholesterolemia during gestation: 1) induces offspring hypoglycemia; 2) does not modify the genetic and protein expressions of SLC2A1 and SLC2A4 (previously GLUT1 and GLUT4) in total placental extract; 3) downregulates the placental SLC5A1 (previously SGLT1) protein expression without affecting its mRNA levels; 4) impairs the translocation of SLC2A1 but not SLC2A4 from cytoplasmatic pool to the cell membrane surface. Then we assume that reduction of offspring birth weight in presence of maternal hypercholesterolemia may be related to the offspring's hypoglycemia and the reduction of the cell surface expression of placental SLC2A1.

glucose, hypercholesterolemia, insulin, placenta, transporters

¹Supported by the National Sciences and Engineering Research of Canada (NSERC).

Correspondence: ²Julie Lafond, Laboratoire de Physiologie Materno-Ftale, Université du Québec à Montréal, Département des Sciences Biologiques, C.P. 8888, Succ. centre-ville, Montréal, Canada H3C 3P8. FAX: 514 987 4647; e-mail: Lafond.julie{at}uqam.ca

This article has been cited by other articles:

				A. N. Sferruzzi-Perri, J. A. Owens, P. Standen, R. L. Taylor, J. S. Robinson, and C. T. Roberts Early Pregnancy Maternal Endocrine Insulin-Like Growth Factor I Programs the Placenta for Increased Functional Capacity throughout Gestation Endocrinology, September 1, 2007; 148(9): 4362 - 4370. [Abstract] [Full Text] [PDF]