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BOR - Papers in Press, published online ahead of print December 13, 2006.
Biol Reprod 2006, 10.1095/biolreprod.106.053462
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BIOLOGY OF REPRODUCTION 76, 719–727 (2007)
DOI: 10.1095/biolreprod.106.053462
© 2007 by the Society for the Study of Reproduction, Inc.

research-article

The Effect of the Presence and Pattern of Luteinizing Hormone Stimulation on Ovulatory Follicle Development in Sheep1

B.K. Campbell 2 3, N.R. Kendall 3, and D.T. Baird 4

School of Human Development,3 University of Nottingham, Queen's Medical Centre, Nottingham NG7 2UH, United Kingdom Department of Reproductive and Developmental Sciences,4 Obstetrics and Gynaecology, University of Edinburgh, Edinburgh EH3 9EW, United Kingdom

ABSTRACT

The aim of this study was to examine the role of LH on the growth of the large preovulatory follicle and its secretion of hormones in sheep. Ewes with ovarian autotransplants were treated with GnRH-antagonist at the time of luteal regression and different LH regimes applied for 60–66 h before administration of an ovulatory stimulus (hCG). In Experiment 1 (N = 24; n = 8), ewes received either no LH or constant or pulsatile infusion of LH at the same dose (1.25 µg/h). In Experiment 2 (N = 12, n = 6), LH was constantly infused at a rate of 1.25 µg or 2.5 µg oLH/h. In Experiment 1, animals receiving either pulsatile or constant LH exhibited increases in estradiol and inhibin A secretion (P < 0.001) and a depression in FSH (P < 0.001) that resembled the normal follicular phase. Similarly in Experiment 2, doubling the dose of LH resulted in a two-fold increase in ovarian estradiol secretion (P < 0.05) but no other changes. All animals receiving LH, regardless of the pattern of stimulation, ovulated and established a normal luteal phase. In contrast, no LH treatment resulted in constant immuno-active LH without pulses, unchanged FSH and inhibin A concentrations (P < 0.05), and basal estradiol secretion (P < 0.001). Morphologically normal large antral follicles were observed in this group and although corpora lutea formed in response to hCG, progesterone profiles were abnormal. In conclusion, these results suggest that LH is an essential requirement for normal ovulatory follicle development and subsequent luteal function and show that a pulsatile mode of LH stimulation is not required by ovulatory follicles.

estradiol, follicle-stimulating hormone, inhibin, inhibin A, luteinizing hormone, ovary, ovulatory follicle, progesterone

FOOTNOTES

1Supported by EEC Grant AIR3 CT 92-0232, MRC programme grant G8929853, and NRK was supported by BBSRC S11749.

Correspondence: 2School of Human Development, University of Nottingham, Floor D East Block, Queen's Medical Centre, Nottingham NG7 2UH, United Kingdom. FAX: 44 115 823 0704; e-mail: bruce.campbell{at}nottingham.ac.uk






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Copyright © 2007 by the Society for the Study of Reproduction.