Biol Reprod Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


BOR - Papers in Press, published online ahead of print December 27, 2006.
Biol Reprod 2006, 10.1095/biolreprod.106.057471
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
76/5/848    most recent
biolreprod.106.057471v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow My Folders
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dragovic, R. A.
Right arrow Articles by Gilchrist, R. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dragovic, R. A.
Right arrow Articles by Gilchrist, R. B.
Agricola
Right arrow Articles by Dragovic, R. A.
Right arrow Articles by Gilchrist, R. B.
BIOLOGY OF REPRODUCTION 76, 848–857 (2007)
DOI: 10.1095/biolreprod.106.057471
© 2007 by the Society for the Study of Reproduction, Inc.

Oocyte-Secreted Factor Activation of SMAD 2/3 Signaling Enables Initiation of Mouse Cumulus Cell Expansion1

Rebecca A. Dragovic , Lesley J. Ritter , Samantha J. Schulz , Fred Amato , Jeremy G. Thompson , David T. Armstrong , and Robert B. Gilchrist 2

Research Centre for Reproductive Health, Discipline of Obstetrics and Gynaecology, Medical School, University of Adelaide, Adelaide, South Australia 5005, Australia

ABSTRACT

Expansion of the mouse cumulus-oocyte complex (COC) is dependent on oocyte-secreted paracrine factors. Transforming growth factor beta (TGFB) superfamily molecules are prime candidates for the cumulus expansion-enabling factors (CEEFs), and we have recently determined that growth differentiation factor 9 (GDF9) alone is not the CEEF. The aim of this study was to examine oocyte paracrine factors and their signaling pathways that regulate mouse cumulus expansion. Using RT-PCR, oocytes were found to express the two activin subunits, Inhba and Inhbb, and activin A and activin B both enabled FSH-induced cumulus expansion of oocytectomized (OOX) complexes. Follistatin, an activin-binding protein, neutralized activin-induced expansion but had no effect on oocyte-induced expansion. The type I receptors for GDF9 and activin are activin receptor-like kinase 5 (ALK5) and ALK4, respectively, both of which activate the same SMAD 2/3 signaling pathway. We examined the requirement for this signaling system using an ALK 4/5/7 inhibitor, SB-431542. SB-431542 completely ablated FSH-stimulated GDF9-, activin A-, activin B-, and oocyte-induced cumulus expansion. Moreover, SB-431542 also antagonized epidermal growth factor-stimulated, oocyte-induced cumulus expansion. Using real-time RT-PCR, SB-431542 also attenuated GDF9-, activin A-, and oocyte-induced OOX expression of hyaluronan synthase 2, tumor necrosis factor alpha-induced protein 6, prostaglandin synthase 2, and pentraxin 3. This study provides evidence that the CEEF is composed of TGFB superfamily molecules that signal through SMAD 2/3 to enable the initiation of mouse cumulus expansion.

activin, cumulus cells, growth factors, oocyte development, signal transduction


FOOTNOTES

1Supported by a National Health and Medical Research Council (NHMRC) of Australia Program Grant and the Research Centre for Reproductive Health. R.A.D. was supported by the Faculty of Health Sciences, Adelaide University, and The Queen Elizabeth Hospital Research Foundation.

Correspondence: 2FAX: 61 08 8303 8177; e-mail: robert.gilchrist{at}adelaide.edu.au




This article has been cited by other articles:


Home page
Am. J. Physiol. Endocrinol. Metab.Home page
Q. Huang, A. P. Cheung, Y. Zhang, H.-F. Huang, N. Auersperg, and P. C. K. Leung
Effects of growth differentiation factor 9 on cell cycle regulators and ERK42/44 in human granulosa cell proliferation
Am J Physiol Endocrinol Metab, June 1, 2009; 296(6): E1344 - E1353.
[Abstract] [Full Text] [PDF]


Home page
Biol. Reprod.Home page
C. X. Yeo, R. B. Gilchrist, and M. Lane
Disruption of Bidirectional Oocyte-Cumulus Paracrine Signaling During In Vitro Maturation Reduces Subsequent Mouse Oocyte Developmental Competence
Biol Reprod, May 1, 2009; 80(5): 1072 - 1080.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
Q. Li, S. A. Pangas, C. J. Jorgez, J. M. Graff, M. Weinstein, and M. M. Matzuk
Redundant Roles of SMAD2 and SMAD3 in Ovarian Granulosa Cells In Vivo
Mol. Cell. Biol., December 1, 2008; 28(23): 7001 - 7011.
[Abstract] [Full Text] [PDF]


Home page
Mol Hum ReprodHome page
Q. Li, L. J. McKenzie, and M. M. Matzuk
Revisiting oocyte-somatic cell interactions: in search of novel intrafollicular predictors and regulators of oocyte developmental competence
Mol. Hum. Reprod., December 1, 2008; 14(12): 673 - 678.
[Abstract] [Full Text] [PDF]


Home page
Physiol. GenomicsHome page
Y. S. Lee, K. E. Latham, and C. A. VandeVoort
Effects of in vitro maturation on gene expression in rhesus monkey oocytes
Physiol Genomics, October 8, 2008; 35(2): 145 - 158.
[Abstract] [Full Text] [PDF]


Home page
Hum Reprod UpdateHome page
K. T. Jones
Meiosis in oocytes: predisposition to aneuploidy and its increased incidence with age
Hum. Reprod. Update, March 1, 2008; 14(2): 143 - 158.
[Abstract] [Full Text] [PDF]


Home page
Hum Reprod UpdateHome page
R. B. Gilchrist, M. Lane, and J. G. Thompson
Oocyte-secreted factors: regulators of cumulus cell function and oocyte quality
Hum. Reprod. Update, March 1, 2008; 14(2): 159 - 177.
[Abstract] [Full Text] [PDF]


Home page
EndocrinologyHome page
S. Cecconi, A. Mauro, G. Capacchietti, P. Berardinelli, N. Bernabo, A. R. Di Vincenzo, M. Mattioli, and B. Barboni
Meiotic Maturation of Incompetent Prepubertal Sheep Oocytes Is Induced by Paracrine Factor(s) Released by Gonadotropin-Stimulated Oocyte-Cumulus Cell Complexes and Involves Mitogen-Activated Protein Kinase Activation
Endocrinology, January 1, 2008; 149(1): 100 - 107.
[Abstract] [Full Text] [PDF]


Home page
Hum ReprodHome page
C. X. Yeo, R. B. Gilchrist, J. G. Thompson, and M. Lane
Exogenous growth differentiation factor 9 in oocyte maturation media enhances subsequent embryo development and fetal viability in mice
Hum. Reprod., January 1, 2008; 23(1): 67 - 73.
[Abstract] [Full Text] [PDF]


Home page
DevelopmentHome page
Y.-Q. Su, K. Sugiura, K. Wigglesworth, M. J. O'Brien, J. P. Affourtit, S. A. Pangas, M. M. Matzuk, and J. J. Eppig
Oocyte regulation of metabolic cooperativity between mouse cumulus cells and oocytes: BMP15 and GDF9 control cholesterol biosynthesis in cumulus cells
Development, January 1, 2008; 135(1): 111 - 121.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2007 by the Society for the Study of Reproduction.