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BOR - Papers in Press, published online ahead of print February 28, 2007.
Biol Reprod 2007, 10.1095/biolreprod.106.057927
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BIOLOGY OF REPRODUCTION 76, 1016–1024 (2007)
DOI: 10.1095/biolreprod.106.057927
© 2007 by the Society for the Study of Reproduction, Inc.

research-article

Molecular Mechanisms of Insulin-Like Growth Factor 1 Promoted Synthesis and Retention of Hyaluronic Acid in Porcine Oocyte-Cumulus Complexes1

Lucie Nemcová 3, Eva Nagyová 3, Michal Petlach 3, Milan Tománek 4, and Radek Procházka 2 3

Academy of Sciences of the Czech Republic,3 Institute of Animal Physiology and Genetics, Libechov 277 21, Czech Republic Research Institute of Animal Production,4 104 01 Prague 10-Uhríneves, Czech Republic

ABSTRACT

The purpose of the present study was to elucidate signaling pathways by which insulin like-growth factor 1 (IGF1) promotes FSH-stimulated synthesis and retention of hyaluronic acid (HA) in pig oocyte-cumulus complexes (OCCs) cultured in serum-free medium. We found that IGF1 had no effects on FSH-stimulated production of cAMP and activation of protein kinase A in the OCCs. Immunoblotting with phospho-specific antibodies showed that FSH moderately phosphorylated v-akt murine thymoma viral oncogene homolog (AKT) and mitogen-activated kinase 3 and 1 (MAPK3/1) in cumulus cells. The exposure of OCCs to both FSH and IGF1 resulted in a significant (P < 0.05) increase in AKT and MAPK3/1 phosphorylation. An inhibitor of phosphoinositide-3-kinase (PIK3), LY 294002, significantly (P < 0.05) reduced the IGF1-enhanced phosphorylation of AKT, and inhibitors of AKT (SH6) and MAPK3/1 (U0126) significantly (P < 0.05) decreased the synthesis and retention of HA stimulated by concomitant exposure of OCCs to both FSH and IGF1. The IGF1-promoted synthesis of HA was not accompanied by an increase in the relative abundance of hyaluronan synthase 2 (HAS2) mRNA in the cumulus cells. We conclude that IGF1 promotes the FSH-stimulated synthesis and retention of HA in pig OCCs by PIK3/AKT- and MAPK3/1-dependent mechanisms.

cumulus cells,, expansion, follicle-stimulating hormone, gamete biology, ovary, pig, signal transduction

FOOTNOTES

1Supported by grant no. 523/04/0574 from the Grant Agency of the Czech Republic and by research project AV0Z50450515 from IAPG. M. Tománek was supported by grant MZE 0002701401 from the Ministry of Agriculture of the Czech Republic.

Correspondence: 2FAX: 420 315 639 510; e-mail: prochazka{at}iapg.cas.cz




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Copyright © 2007 by the Society for the Study of Reproduction.