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BOR - Papers in Press, published online ahead of print April 11, 2007.
Biol Reprod 2007, 10.1095/biolreprod.106.056556
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BIOLOGY OF REPRODUCTION 77, 181–188 (2007)
DOI: 10.1095/biolreprod.106.056556
© 2007 by the Society for the Study of Reproduction, Inc.

Changes in Lipids Containing Long- and Very Long-Chain Polyunsaturated Fatty Acids in Cryptorchid Rat Testes1

Natalia E. Furland 2, Eduardo N. Maldonado , Pablo Ayuza Aresti , and Marta I. Aveldaño 

Institute for Biochemical Research of Bahía Blanca, National Research Council and University of the South, 8000-Bahía Blanca, Argentina

ABSTRACT

The aim of the present study was to examine the effects of experimental cryptorchidism on rat testicular phospholipids and neutral lipids that contain long-chain (C18-C22) and very long-chain (VLC) (C24-C32) polyunsaturated fatty acids (PUFA). The weight of the cryptorchid testis was nearly half that of the contralateral control at postsurgical Days 7–10 owing to the depletion of germ cells. Concomitantly, the amounts of major glycerophospholipids (GPL) and sphingomyelin (SM) per testis decreased. Both these lipids lost their characteristic long-chain and very long-chain PUFA, notably 22:5n-6 and 28:4n-6, respectively, which suggests that these species are linked to the membranes of germ cells. In contrast, the amounts and concentrations of triglycerides (TG; triacylglycerols and 1-O-alkyl-2,3-diacylglycerols) and cholesterol esters (CE) increased several fold in the surviving cells (mainly Sertoli cells) in the cryptorchid testis. All these neutral lipids, but especially CE, accumulated large amounts of the major PUFA of the testis, 22:5n-6, as well as pentaenes with longer carbon chains (i.e., 24:5n-6 in TG and 28:5n-6 in CE). This accretion suggests that neutral lipids may store preformed PUFA coming from dying germ cell GPL and also VLCPUFA no longer needed as a source of PUFA destined to assemble new germ cell GPL. The lipid adjustments observed in cryptorchidism suggest a possible role for Sertoli cell CE in the turnover and conservation of PUFA within seminiferous tubules.

Sertoli cells, spermatid, spermatogenesis, stress, testis

FOOTNOTES

1This study was supported by grants from CONICET, SGCyT-UNS, and FONCyT, Argentina.

Correspondence: 2Natalia E. Furland, INIBIBB, UNS-CONICET, Centro Regional de Investigaciones Básicas y Aplicadas de Bahía Blanca, CC 857, 8000-Bahía Blanca, Argentina. FAX: 54 291 486 1200; e-mail: nfurland{at}criba.edu.ar






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Copyright © 2007 by the Society for the Study of Reproduction.