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An Impaired Breeding Phenotype in Mice with a Genetic Deletion of Beta-2 Microglobulin and Diminished MHC Class I Expression: Role in Reproductive Fitness¹

Department of Experimental Biology,⁴ Huntingdon Life Sciences Ltd., Alconbury, Huntingdon, Cambridgeshire PE28 4HS, England Department of Biochemistry and Microbiology,⁵ Nelson Mandela Metropolitan University, Summerstrand South Campus, Port Elizabeth 6031, South Africa Histology Department,⁶ Faculty of Medicine, Ain Shams University, Cairo, Egypt University Medicine Berlin,⁷ Charité Centrum 12 Internal Medicine and Dermatology, Campus Virchow, 13353 Berlin, Germany Department of Biological Sciences,⁸ University of Essex, Colchester C04 3SQ, England

ABSTRACT

Beta-2 microglobulin (B2M) plays a pivotal role in the biology of mammals, including its association with major histocompatibility complex (MHC) Class I gene products. The latter molecules have been shown to affect reproduction in both mice and humans, although the exact mechanism is still unknown. Here we report the results of a longitudinal study of the reproductive performance of a genetically modified B2m deficient mouse strain with low MHC Class I expression. Our data show that this mouse strain has an impaired reproductive performance. However, the mice superovulate well and show a normal estrous cycle. Breeding studies from crosses between the transgenic mice and the wild-type parental strain show that B2m deficient mice have a significantly lower frequency of mating than the control B2m^+/+ mice. In addition, the litter size and weaning success of B2m deficient mice were lower than the control. Perinatal lethality of the B2m deficient offspring was also inflicted by cannibalism of the young pups by the B2m deficient female. The impaired breeding phenotype (IBP) can be reversed by reintroducing the B2m gene in F1 heterozygous B2m^+/– animals; thus the presence of B2M confers a normal breeding pattern. The acquisition of an impaired breeding phenotype (IBP) as a result of the knockout of B2m directly implicates B2M in the reproductive cycle of mice and raises the possibility of an effect of B2M on the reproduction of other mammals.

beta-2 microglobulin,, female reproductive tract,, fetus,, MHC (H-2),, odor discrimination,, odortypes

³These authors contributed equally to this work.

¹Supported by the Wellcome Trust. S.M.B. is supported by Charité through the Habilitation Fellowship program. S.M.B. and P.C.A. are part of the EMBIC Network of Excellence, cofinanced by the European Commission through the FP6 framework program "Life Science, Genomics and Biotechnology for Health."

Correspondence: ²N. Fernández, Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester CO4 3SQ, England. FAX: 44 1206 873330; e-mail: nelson{at}essex.ac.uk

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