Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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BOR - Papers in Press, published online ahead of print April 11, 2007.
Biol Reprod 2007, 10.1095/biolreprod.106.059261
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BIOLOGY OF REPRODUCTION 77, 365–375 (2007)
DOI: 10.1095/biolreprod.106.059261
© 2007 by the Society for the Study of Reproduction, Inc.

The Role of Aquaporin 3 in the Movement of Water and Cryoprotectants in Mouse Morulae1

Keisuke Edashige 2 3, Satoshi Ohta 3, Mitsunobu Tanaka 3, Tatsunaga Kuwano 3, Delgado M. Valdez, Jr. 3, Takao Hara 3, Bo Jin 3, Sei-ichi Takahashi 3, Shinsuke Seki 3, Chihiro Koshimoto 4, and Magosaburo Kasai 3

Laboratory of Animal Science,3 College of Agriculture, Kochi University, Nankoku, Kochi 783-8502, Japan Frontier Science Research Center,4 University of Miyazaki, Kiyotake, Miyazaki 889-1692, Japan

ABSTRACT

The permeability to water and cryoprotectants of the plasma membrane is crucial to the successful cryopreservation of embryos. Previously, we have shown in mouse morulae that water and glycerol move across the plasma membrane by facilitated diffusion, and we have suggested that aquaporin 3 plays an important role in their movement. In the present study, we clarify the contribution of aquaporin 3 to the movement of water and various cryoprotectants in mouse morulae by measuring the Arrhenius activation energies for permeability to cryoprotectants and water, through artificial expression of aquaporin 3 using Aqp3 cRNA in mouse oocytes, and by suppressing the expression of aquaporin 3 in morulae by injecting double-stranded RNA of Aqp3 at the one-cell zygote stage. The results show that aquaporin 3 plays an important role in the facilitated diffusion of water, glycerol, and ethylene glycol, but not of acetamide and dimethylsulfoxide. On the other hand, in a propylene glycol solution, aquaporin 3 in morulae transported neither propylene glycol nor water by facilitated diffusion, probably because of strong water-solute interactions. These results provide important information for understanding the permeability of the plasma membrane of the mouse embryo.

developmental biology,, embryo,, ovum

FOOTNOTES

1Supported by grants-in-aid for scientific research from the Ministry of Education, Culture, Sports, Science and Technology; and the Ministry of Health, Labour and Welfare of Japan.

Correspondence: 2Correspondence. FAX: 81 88 864 5200; e-mail: keisuke{at}kochi-u.ac.jp






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Copyright © 2007 by the Society for the Study of Reproduction.