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BOR - Papers in Press, published online ahead of print May 30, 2007.
Biol Reprod 2007, 10.1095/biolreprod.106.059451
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BIOLOGY OF REPRODUCTION 77, 517–525 (2007)
DOI: 10.1095/biolreprod.106.059451
© 2007 by the Society for the Study of Reproduction, Inc.

Regulation of 17-Beta Hydroxysteroid Dehydrogenase Type 2 in Human Placental Endothelial Cells1

Emily J Su 3, You-Hong Cheng 4, Robert T Chatterton 4 5, Zhi-Hong Lin 4, Ping Yin 4, Scott Reierstad 4, Joy Innes 4, and Serdar E Bulun 2 4

Divisions of Maternal-Fetal Medicine3 and Reproductive Biology Research,4 Department of Obstetrics and Gynecology, and Department of Physiology,5 Northwestern University, Chicago, Illinois 60611

ABSTRACT

17-beta hydroxysteroid dehydrogenase type 2 (HSD17B2) oxidizes estradiol to estrone, testosterone to androstenedione, and 20 alpha-dihydroprogesterone to progesterone. HSD17B2 is highly expressed in human placental tissue where it is localized to placental endothelial cells lining the fetal compartment. The aim of this study was to investigate the effects of potential regulatory factors including progesterone, estradiol, and retinoic acid (RA) onHSD17B2 expression in primary human placental endothelial cells in culture.HSD17B2 mRNA expression was not regulated by progesterone, the progesterone agonist R5020, or estradiol treatment. RA significantly induced HSD17B2 mRNA levels and enzyme activity in a dose- and time-dependent manner. Maximal stimulation occurred at Hour 48 at an RA concentration of 10–6 M. Both retinoic acid receptor alpha (RARA) and retinoid X receptor alpha (RXRA) were readily detected by immunoblotting in isolated placental endothelial cells. RNA interference directed against RARA or RXRA led to reduced basal levels of HSD17B2 mRNA levels and significantly abolished RA-stimulated HSD17B2 expression. Together, these data indicate that regulation of HSD17B2 mRNA levels and enzymatic activity by RA in the placenta is mediated by RARA and RXRA.

placenta, pregnancy, steroid hormones, steroid hormone receptors

FOOTNOTES

1Supported by the Northwestern Memorial Foundation Young Investigator's Award and NIH grant HD40093.

Correspondence: 2Serdar E. Bulun, 303 E. Superior Street, Lurie Building 4-250, Chicago, IL 60611. FAX: 312 926 6675; e-mail: s-bulun{at}northwestern.edu




This article has been cited by other articles:


Home page
 J. Clin. Endocrinol. Metab.Home page
Y.-H. Cheng, P. Yin, Q. Xue, B. Yilmaz, M. I. Dawson, and S. E. Bulun
Retinoic Acid (RA) Regulates 17{beta}-Hydroxysteroid Dehydrogenase Type 2 Expression in Endometrium: Interaction of RA Receptors with Specificity Protein (SP) 1/SP3 for Estradiol Metabolism
J. Clin. Endocrinol. Metab., May 1, 2008; 93(5): 1915 - 1923.
[Abstract] [Full Text] [PDF]


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Copyright © 2007 by the Society for the Study of Reproduction.