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Changes in Histone Acetylation During Postovulatory Aging of Mouse Oocyte¹

State Key Laboratory of Reproductive Biology,⁴ Institute of Zoology, and Graduate School,⁵ Chinese Academy of Sciences, Beijing 100080, China

ABSTRACT

Because some animals and human beings potentially engage in sexual activity at any day of the menstrual cycle, this may cause fertilization of postovulatory aged oocytes, which result in decreased potential of embryo development and longevity of offspring. To investigate the involvement of histone acetylation in the function of postovulatory aging, we examined the changes of histone acetylation by immunostaining with specific antibodies against various acetylated lysines on histones H3 and H4. We found that the acetylation levels of lysine 14 on histone H3 and lysines 8 and 12 on histone H4 in mouse oocytes were gradually increased during in vivo and in vitro postovulatory aging. Furthermore, the acetylation levels on these sites were markedly decreased or increased when the process of postovulatory aging was artificially delayed or accelerated, respectively. These results indicated that the gradual acetylation on some lysines of histones H3 and H4 is one of the phenomena in the process of postovulatory aging. Moreover, raising the level of histone acetylation by trichostatin A can accelerate the progression of postovulatory aging, suggesting that alteration of the acetylation on histones H3 and H4 can affect the progression of postovulatory aging in mouse oocytes.

aging,, gamete biology, histone acetylation, mouse, oocyte development, postovulatory aging, trichostatin A

³Current address: Peking University Third Hospital, 49 North Garden Rd., Haidian, Beijing 100083, China.

¹Supported by the Special Funds for Major State Basic Research Project of China (A1902164).

Correspondence: ²Da-Yuan Chen, State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 25 Beisihuan Xi Rd., Haidian, Beijing 100080, China. FAX: 86 10 64807316; e-mail: chendy{at}ioz.ac.cn

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