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BOR - Papers in Press, published online ahead of print October 3, 2007.
Biol Reprod 2007, 10.1095/biolreprod.107.063537
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BIOLOGY OF REPRODUCTION 78, 176–183 (2008)
DOI: 10.1095/biolreprod.107.063537
© 2008 by the Society for the Study of Reproduction, Inc.

Regulation of Pcsk6 Expression During the Preantral to Antral Follicle Transition in Mice: Opposing Roles of FSH and Oocytes1

Francisco J Diaz , Koji Sugiura , and John J Eppig 2

The Jackson Laboratory, Bar Harbor, Maine 04609

ABSTRACT

Several secreted products of the TGFbeta superfamily have important roles during follicular development and are produced by both oocytes and somatic cells (granulosa and theca) in the follicle. The proprotein convertases are a family of seven known proteins that process TGFbeta ligands and other secreted products to their mature active form. The present study examined the regulation of steady-state levels of Pcsk6 mRNA, which encodes a convertase protein known to process members of the TGFbeta superfamily, during mouse follicular development. Pcsk6 mRNA and protein were expressed in preantral but not cumulus or mural granulosa cells. Pcsk6 mRNA levels in preantral granulosa cells were not regulated by growing oocytes of preantral follicles, but were elevated by FSH. Furthermore, Pcsk6 mRNA in preantral granulosa cells was potently suppressed by factor(s) secreted by fully grown oocytes from antral follicles, in part through SMAD2/3-mediated pathways. Oocytes acquired the ability to suppress the steady-state levels of Pcsk6 mRNA in granulosa cells during the preantral to antral follicle transition. Suppression of Pcsk6 mRNA by oocytes could reflect a change in the mechanism(s) regulating the activity of members of the TGFbeta superfamily.

cumulus cells, convertase, follicle, follicular development, gamete biology, granulosa, granulosa cells, oocyte, oocyte development

FOOTNOTES

1This work was supported by NIH HD23839.

Correspondence: 2John J. Eppig, The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609. FAX: 207 288 6073; e-mail: john.eppig{at}jax.org






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Copyright © 2008 by the Society for the Study of Reproduction.