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BOR - Papers in Press, published online ahead of print March 5, 2008.
Biol Reprod 2008, 10.1095/biolreprod.107.064881
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BIOLOGY OF REPRODUCTION 79, 17–25 (2008)
DOI: 10.1095/biolreprod.107.064881
© 2008 by the Society for the Study of Reproduction, Inc.

research-article

Disruption of Lipid Rafts Induces Gonadotropin Release in Ovine Pituitary and LbetaT2 Gonadotroph Cells1

E Robin , J Cognié , F Foulon-Gauze , J Fontaine , and X Cayla 2

UMR Physiologie de la Reproduction et des Comportements, INRA/CNRS/Université Tours/Haras Nationaux, 37380 Nouzilly, France

ABSTRACT

In order to better understand the cellular mechanisms underlying LH and FSH secretion, we have addressed the contribution of lipid rafts to the secretion of gonadotropins. We used methyl-beta-cyclodextrin (MbetaCD), a cholesterol-sequestering agent, on an LbetaT2 murine gonadotroph cell line and on primary cultures of ovine pituitary cells. We found that in both systems, cholesterol depletion by MbetaCD induced a fast and substantial release of LH in the absence of natural stimulation by GnRH. In ovine pituitary cells, MbetaCD-mediated LH release was shown to be independent of protein synthesis. Twenty-four hours after MbetaCD treatment, there was no loss of cell viability and full recovery of LH secretory capabilities, as determined by GnRH or MbetaCD treatment. In addition, our data suggest the existence of a pool of LH that is not released by GnRH treatment but that is released by MbetaCD treatment. Finally, in ovine pituitary cells, MbetaCD treatment induced FSH secretion. Importantly, these in vitro data are supported by in vivo studies, because MbetaCD injected into the pituitary glands of anaesthetized sheep reproducibly induced a peak of LH release.

exocytosis, follicle-stimulating hormone, lipid rafts, luteinizing hormone, pituitary

FOOTNOTES

1Supported by Conseil régional Région Centre/Institut National de la Recherche Agronomique. E.R. is supported by a PhD fellowship from Institut National de la Recherche Agronomique/Région Centre.

Correspondence: 2FAX: 33 24 742 7743; e-mail: cayla{at}tours.inra.fr






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Copyright © 2008 by the Society for the Study of Reproduction.