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This Article Full Text Full Text (PDF) [Supplemental Tables] All Versions of this Article: 79/3/413    most recent biolreprod.108.067959v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Rengaraj, D. Articles by Han, J. Y. PubMed PubMed Citation Articles by Rengaraj, D. Articles by Han, J. Y. Agricola Articles by Rengaraj, D. Articles by Han, J. Y.

Testis-Specific Novel Transcripts in Chicken: In Situ Localization and Expression Pattern Profiling During Sexual Development¹

Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, 151-921 Seoul, Korea

ABSTRACT

Tissue-specific novel transcripts expressed during sexual development were examined by RT-PCR, quantitative RT-PCR (qRT-PCR), and in situ hybridization to provide data for chicken genomics. Public databases for transcript data have been constructed with known and unknown sequences of various tissues from different animals. However, the expression patterns and functions of the transcripts are less known. From the The Institute for Genomics Research Gallus gallus library, we examined 291 tentative consensus (TC) sequences that assembled 100% with transcripts by RT-PCR during male and female sexual development from Embryonic Day 6 to 25 wk of age. We found 85 TC sequences that were specific to testicular development; of these, 43 TC sequences were exclusively upregulated in 25-wk-old testis. Another 52 TC sequences were not specific to one tissue, but occurred in the testis and ovary at different developmental ages. Twelve testis-specific TC sequences upregulated in 25-wk-old testis were randomly selected and further examined with qRT-PCR. For precise localization, these 12 testis-specific TC sequences were examined by in situ hybridization with 25-wk-old adult testis. Six TC sequences were strongly expressed in secondary spermatocytes and haploid spermatids until spermatozoa release. Another six TC sequences were differentially expressed in the adluminal compartment of seminiferous tubules. Among the testis-specific TC sequences, TC120901 is a known gene, phospholipase C, zeta (PLCZ1). Our data provide potential insight into gene expression and genomic information on novel transcripts that are important to avian reproduction.

chicken, in situ hybridization, spermatid, spermatogenesis, TC sequences, testis, testis-specific transcripts

¹ Supported by a grant from the BioGreen 21 Program, Rural Development Administration, Republic of Korea, and by the Brain Korea 21 Project of the Ministry of Education and Research Institute for Agriculture and Life Sciences, Seoul National University.

Correspondence: ²FAX: 82 2 874 4811; e-mail: jaehan{at}snu.ac.kr