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BOR - Papers in Press, published online ahead of print August 13, 2008.
Biol Reprod 2008, 10.1095/biolreprod.108.070474
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biolreprod.108.070474v1
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BIOLOGY OF REPRODUCTION 79, 906–913 (2008)
DOI: 10.1095/biolreprod.108.070474
© 2008 by the Society for the Study of Reproduction, Inc.

UBE2I (UBC9), a SUMO-Conjugating Enzyme, Localizes to Nuclear Speckles and Stimulates Transcription in Mouse Oocytes1

Motomasa Ihara , Paula Stein , and Richard M. Schultz  2

Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6018

ABSTRACT

Sumoylation is a posttranslational modification in which SUMO (small ubiquitin-related modifier) proteins are covalently attached to their substrates. In vertebrates, developmental roles for sumoylation have been studied, but the function of sumoylation during mammalian oocyte growth and maturation is not known. As a prelude to conducting studies on the role of sumoylation during oocyte development, we analyzed the temporal and spatial pattern of expression of UBE2I, a SUMO-conjugating E2 enzyme. Immunocytochemical analysis of UBE2I revealed a punctate nuclear staining pattern, with transcriptionally quiescent, fully grown, GV-intact oocytes having larger UBE2I-containing bodies than transcriptionally active, meiotically incompetent growing oocytes. Inhibiting transcription in incompetent oocytes resulted in an increase in the size of the UBE2I-containing bodies. Overexpression of either wild-type UBE2I or catalytically inactive UBE2I resulted in an increase in the size of the UBE2I-containing bodies but also an increase in BrUTP incorporation, suggesting that transcriptional activation by UBE2I is independent of its catalytic activity. Although UBE2I-containing bodies did not completely colocalize with SUMO1 or SUMO2 and SUMO3, which were localized mainly on the nuclear membrane and in the nucleoplasm, UBE2I strikingly colocalized with SFRS2, which is a component of nuclear speckles and critical for mRNA processing. These results suggest a novel function for UBE2I and therefore sumoylation in gene expression..

nuclear speckle, oocyte development, oogenesis, SUMO, transcription, UBE2I

FOOTNOTES

1This work was supported by a grant from the National Institutes of Health (HD22681) to R.M.S. and the Naito Foundation to M.I.

Correspondence: 2Richard M. Schultz, Department of Biology, 204B Lynch Laboratory, University of Pennsylvania, 433 South University Ave., Philadelphia, PA 19104-6018. FAX: 215 898 8780; e-mail: rschultz{at}sas.upenn.edu






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Copyright © 2008 by the Society for the Study of Reproduction.