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BOR - Papers in Press, published online ahead of print August 27, 2008.
Biol Reprod 2008, 10.1095/biolreprod.108.070268
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biolreprod.108.070268v1
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BIOLOGY OF REPRODUCTION 79, 1054–1061 (2008)
DOI: 10.1095/biolreprod.108.070268
© 2008 by the Society for the Study of Reproduction, Inc.

Murine Pregnancy-Specific Glycoprotein 23 Induces the Proangiogenic Factors Transforming-Growth Factor Beta 1 and Vascular Endothelial Growth Factor A in Cell Types Involved in Vascular Remodeling in Pregnancy1

Julie A. Wu , Briana L. Johnson , Yongqing Chen , Cam T. Ha , and Gabriela S. Dveksler 2

Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814

ABSTRACT

Haemochorial placentation is a unique physiological process in which the fetal trophoblast cells remodel the maternal decidual spiral arteries to establish the fetoplacental blood supply. Pregnancy-specific glycoproteins (PSGs) are members of the carcinoembryonic antigen family. PSGs are produced by the placenta of rodents and primates and are secreted into the bloodstream. PSG23 is one of 17 members of the murine PSG family (designated PSG16 to PSG32). Previous studies determined that PSGs have immunoregulatory functions due to their ability to modulate macrophage cytokine secretion. Here we show that recombinant PSG23 induces transforming growth factor (TGF) beta1, TGFB1, and vascular endothelial growth factor A (VEGFA) in primary murine macrophages and the macrophage cell line RAW 264.7 cells. In addition, we identified new cell types that responded to PSG23 treatment. Dendritic cells, endothelial cells, and trophoblasts, which are involved in maternal vasculature remodeling during pregnancy, secreted TGFB1 and VEGFA in response to PSG23. PSG23 showed cross-reactivity with human cells, including human monocytes and the trophoblast cell line, HTR-8/SVneo cells. We analyzed the binding of PSG23 to the tetraspanin CD9, the receptor for PSG17, and found that CD9 is not essential for PSG23 binding and activity in macrophages. Overall these studies show that PSGs can modulate the secretion of important proangiogenic factors, TGFB1 and VEGFA, by different cell types involved in the development of the placenta.

angiogenesis, mouse pregnancy-specific glycoproteins

FOOTNOTES

1Supported by grant R01HD035832 from the National Institutes of Health.

Correspondence: 2Gabriela S. Dveksler, Department of Pathology, Room #B3125, USUHS, 4301 Jones Bridge Road, Bethesda, Maryland 20814. FAX: 301 295 1640; e-mail: gdveksler{at}usuhs.mil






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Copyright © 2008 by the Society for the Study of Reproduction.