Biol Reprod Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

BOR - Papers in Press, published online ahead of print August 27, 2008.
Biol Reprod 2008, 10.1095/biolreprod.108.068478
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
79/6/1192    most recent
biolreprod.108.068478v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow My Folders
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kim, K.-H.
Right arrow Articles by Lee, K.-A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kim, K.-H.
Right arrow Articles by Lee, K.-A.
Agricola
Right arrow Articles by Kim, K.-H.
Right arrow Articles by Lee, K.-A.
BIOLOGY OF REPRODUCTION 79, 1192–1201 (2008)
DOI: 10.1095/biolreprod.108.068478
© 2008 by the Society for the Study of Reproduction, Inc.

SEBOX Is Essential for Early Embryogenesis at the Two-Cell Stage in the Mouse1

Kyeoung-Hwa Kim , Eun-Young Kim , and Kyung-Ah Lee 2

Graduate School of Life Science and Biotechnology, Pochon CHA University College of Medicine, CHA Research Institute, Fertility Center, CHA General Hospital, Seoul 135-081, Korea

ABSTRACT

Previously, we found high levels of skin-embryo-brain-oocyte homeobox (Sebox) gene expression in germinal vesicle (GV)-stage oocytes. The objective of the present study was to determine the role played by SEBOX in oocyte maturation and early embryogenesis using RNA interference (RNAi). Microinjection of Sebox double-stranded RNA into GV oocytes resulted in a marked decrease in Sebox mRNA and protein expression. However, Sebox RNAi affects neither oocyte maturation rate nor morphological characteristics, including spindle and chromosomal organization of metaphase II oocytes. In addition, Sebox RNAi had no discernible effect on the activities of M-phase promoting factor or mitogen-activated protein kinase. In contrast, microinjection of Sebox double-stranded RNA into pronuclear-stage embryos resulted in holding embryo development at the two-cell (84.9%) and the four- and eight-cell (15.1%) stages. We concluded that Sebox is a new addition to maternal effect genes that produced and stored in oocytes and function in preimplantation embryo development.

oocyte maturation, preimplantation embryogenesis, RNA interference, Sebox

FOOTNOTES

1This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korean government (R01-2007-000-20451-0) and by a grant (SC4021) from the Stem Cell Research Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology, Republic of Korea.

Correspondence: 2Kyung-Ah Lee, Graduate School of Life Science and Biotechnology, Pochon CHA University College of Medicine, 606-13 Yeoksam-1-dong, Gangnam-gu, Seoul 135-081, Korea. FAX: 822 563 2028; e-mail: leeka{at}ovary.co.kr






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2008 by the Society for the Study of Reproduction.