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Biology of Reproduction, Vol 9, 420-425, Copyright © 1973 by Society for the Study of Reproduction

An Improved Method for the Artificial Insemination of the Mouse (Mus musculus)

PETER A. LECKIE 1, J. GARY WATSON 1, , and STERLING CHAYKIN 1

1 Department of Biochemistry and Biophysics, University of California, Davis, California 95616


A technique has been devised for the artificial insemination of mice (C3HeB/FeJ) which does not require the participation of a vasectomized male. The use of an artificial penis and a vaginal tampon have been shown to be satisfactory substitutes for the vasectomized males. When both the artificial penis and the vaginal tampon were used 69-87% of the inseminated animals successfully implanted embryos. The implantation percentages when the artificial penis and the vaginal tampon were used separately were 31 and 50, respectively.

Accepted on May 7, 1973




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Copyright © 1973 by the Society for the Study of Reproduction.