HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 74/4/684    most recent biolreprod.105.049312v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal My Folders Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yang, W.-X. Articles by Sperry, A. O. Search for Related Content PubMed PubMed Citation Articles by Yang, W.-X. Articles by Sperry, A. O. Agricola Articles by Yang, W.-X. Articles by Sperry, A. O.

The Molecular Motor KIFC1 Associates with a Complex Containing Nucleoporin NUP62 That Is Regulated During Development and by the Small GTPase RAN¹

Department of Anatomy and Cell Biology,³ Brody School of Medicine at East Carolina University, Greenville, North Carolina 27834 Department of Biology,⁴ College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310029, China

ABSTRACT

KIFC1 is a C-terminal kinesin motor associated with the nuclear membrane and acrosome in round and elongating spermatids. This location in developing spermatids is consistent with possible roles in acrosome elongation and manchette motility or both. Here we describe the association of the KIFC1 motor with a complex containing the nucleoporin NUP62. Formation of this complex is developmentally regulated, being absent before puberty and appearing only after nuclear elongation has begun. In addition, the integrity of this complex is dependent on GTP hydrolysis and the GTP state of the small GTPase RAN. Concomitant with the association of this motor with the NUP62-containing complex is an apparent reorganization of the nuclear pore with loss of NUP62 from larger complexes containing other nucleoporins. The association of KIFC1 with a component of the nuclear membrane is more consistent with a role for this motor in acrosome/manchette transport along the nuclear membrane than for a role for this motor in transport of vesicles along the outer face of the manchette.

gamete biology, manchette, molecular motor, nuclear pore, RAN, sperm, spermatid, spermatogenesis, testis

¹ Supported in part by NIH GM60628 (A.O.S.).

² Correspondence: FAX: 252 744 2850; sperrya{at}ecu.edu

This article has been cited by other articles:

				J. Zhou, Y.-R. Du, W.-H. Qin, Y.-G. Hu, Y.-N. Huang, L. Bao, D. Han, A. Mansouri, and G.-L. Xu RIM-BP3 is a manchette-associated protein essential for spermiogenesis Development, February 1, 2009; 136(3): 373 - 382. [Abstract] [Full Text] [PDF]

				S. Nath, E. Bananis, S. Sarkar, R. J. Stockert, A. O. Sperry, J. W. Murray, and A. W. Wolkoff Kif5B and Kifc1 Interact and Are Required for Motility and Fission of Early Endocytic Vesicles in Mouse Liver Mol. Biol. Cell, May 1, 2007; 18(5): 1839 - 1849. [Abstract] [Full Text] [PDF]