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Khoo et al., Biol Reprod 73 (6) 1147-1156.
BIOLOGY OF REPRODUCTION 74, 448–448 (2006)
DOI: 10.1095/biolreprod.105.050088
© 2006 by the Society for the Study of Reproduction, Inc.
Melissa L.M. Khoo, Leon R. McQuade, Murray S.R. Smith, Justin G. Lees, Kuldip S. Sidhu, and Bernard E. Tuch. Growth and Differentiation of Embryoid Bodies Derived from Human Embryonic Stem Cells: Effect of Glucose and Basic Fibroblast Growth Factor. Biol Reprod 2005 73: 1147–1156. Published online ahead of print 3 August 2005, DOI: 10.1095/biolreprod.104.036673
The first line of the Results section is missing in the print version of this paper. The complete paragraph is reprinted below:
RESULTS
Morphology and Histomorphology of EBs
A representative set of images of EBs cultured in 5.5 mM glucose media is depicted in Figure 1. Typically, dissected hES colony pieces formed rounded discrete structures and acquired an EB appearance within 12 h (Fig. 1, A and D). The external morphology of EBs was in some cases highly variable, even between EBs growing under the same conditions. Continued growth of EBs in suspension culture resulted in the acquisition of a cystic appearance Fig. 1C) and increased diameter.
Also, figure 6 contains an error in the color labeling of laminin and collagen type IV. Laminin staining should be referenced as "green" in the figure and collagen type IV as "red." The corrected legend and figure appear below in their entirety.
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FIG. 6. PAS and immunofluorescent staining of EBs at Days 28, 60, and 104. A) Day 60 EB stained with hematoxylin and eosin and PAS. A region of dedifferentiating neuroectoderm is observed at the arrow, whereas a region of endodermal epithelial lining is observed at the periphery (arrowheads). The core of the EB (*) is developing hyaline cartilage. B) High-power image of the epithelium at the periphery of a Day 60 EB (see A). The goblet cells stain magenta (arrowheads). Compare with D. C) Day 28 EB (without FGF2 supplementation) stained for nestin expression (red). Nestin is only expressed in the neuroepithelial region (arrowheads). D) Day 60 EB stained for AFP (green). The epithelium is similar to that stained positively with PAS Fig. 2F), confirming endodermal lineage. E) Day 60 EB stained for laminin (green) and collagen type IV (red). The collagen type IV is in the core of the EB. F) Day 104 EB stained for laminin (green) and collagen type IV (red). The collagen stain is in the core of the EB, and this region was described as cartilage (Fig. 3C). C–F were counterstained with DAPI (blue)
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