Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - "Average testis weight (mg) during the first wave of spermatogenesis in hpg and WT control mice. P-value <0.001 is represented by a star. (n = 26 for all days). Hpg3-27 or WT3-27 corresponds to the testis weight of hpg mouse or WT mouse at each of the postnatal days indicated."
• Supplemental Figure 2 - "H and E staining of testis sections over the first wave of spermatogenesis in the hpg mouse and WT age matched control at postnatal days 3, 8, 13, 19 and 27. In WT, this corresponds with the appearance of early spermatocytes at day 8, late spermatocytes at day 13, round spermatids at day 19 and elongated spermatids and mature sperm visible in the tubule lumen by day 27. In the hpg testis early spermatocytes can be seen by day 8 and subsequent stages but there is no further progression of spermatogenesis. Mean seminiferous tubule diameter is shown in the lower right corner of each picture. (8 &mgr; m sections were stained with haematoxylin and eosin, all photos were taken at x 40 magnification)"
• Supplemental Figure 3 - "Labeling of Leydig cells by TUNEL assay.The number of Leydig cells positive in the TUNEL assay is very variable from one section to another and the absolute number stays relatively small in the hpg mouse. No apoptotic Leydig cells were observed at postnatal day 8, but some were visible at day 3, 13, and at the adult stage. Very few apoptotic Leydig cells were observed in the WT mouse testis. (All photos were taken at x 100 magnification.)"
• Supplemental Table 5 - "The complete list of Log2(ratio (XX^Sxrb/WT)) of expression values in XX^Sxrb (no germ cells) and WT testis RNA for all the genes present on the mmcDNAv1 array. This permits assignment of transcripts to a given cellular compartment of the testis. Up-regulated transcripts are probably localized in the somatic compartment while down-regulated transcripts are probably localized to the germ cell compartment. Transcripts showing no clear up- or down-regulation are likely to have a more ubiquitous pattern of expression in the testis."
• Supplemental Table 2 - "The complete list of genes which were deregulated at day 8 in the hpg mouse testis compared to the age matched WT testis. The data were obtained from hybridization to the mmcDNAv1 array. Data are presented as the Log2 (ratio [hpg/WT])."
• Supplemental Table 3 - "The complete list of genes which were deregulated at day 13 in the hpg mouse testis compared to the age matched WT testis. The data were obtained from hybridization to the mmcDNAv1 array. Data are presented as the Log2 (ratio [hpg/WT])."
• Supplemental Table 4 - "The complete list of genes which were deregulated at day 13 in the hpg mouse testis compared to the age matched WT testis. The data were obtained from hybridization to the MEEBO array. Data are presented as the Log2 (ratio [hpg/WT])."
• Supplemental Table 1 - "The complete list of genes which were deregulated at day 3 in the hpg mouse testis compared to the age matched WT testis. The data were obtained from hybridization to the mmcDNAv1 array. Data are presented as the Log2 (ratio [hpg/WT])."