Supplemental Data

Files in this Data Supplement:

• [Supplemental Figure 2] - "HPLC of metabolites formed by lamprey testis tissue incubated with tritiated progesterone (P). At least five metabolites were present in the crude extract of which three appeared to bind well to proteins in testis cytosol but with lower affinity to liver cytosol. These peaks had HPLC elution times that corresponded to the expected elution positions (arrows) of 15alpha-hydroxyprogesterone (15alpha-P), androstenedione (Ad), and P. The putative ³H-Ad in the 51 min fraction mostly co-migrated with standard Ad on Thin Layer Chromatography (TLC). There was evidence for some other compounds in this fraction (i.e. small bands on TLC), but when incubated overnight with testis cytosol, commercial ³H-Ad label was not converted to any other steroid (Insert), confirming that Ad, and not a metabolite of Ad, was likely to be the main ligand for the testis cytosol binding moiety."
• [Supplemental Figure 4] - "Association and dissociation kinetics of the cytosolic moiety from lamprey testis. For association kinetics (a), T_1/2 = 11.78 ± 4.10 min, n = 3. For dissociation kinetics (b), T_1/2 = 294.32 ± 29.77 min, n = 3."
• [Supplemental Figure 1] - "Photographs illustrating a secondary sex characteristic in the sea lamprey. The "rope" anterior to the dorsal fins of the lampreys gets larger as the male matures. In immature males (a) the rope tissue is not discernable, but in mature males (b) it is easily observed."
• [Supplemental Figure 5] - "Representative saturation curve (a) and hyperbolic regression curve (b) for the binding of tritiated androstenedione (Ad) to nuclear fractions from testicular tissue. The saturation curve shows total binding (B_T), non-specific binding (B_NS), and specific binding (B_S); abbreviations are K_D, dissociation constant; B_max, maximum binding capacity of tissue; F, free (i.e. unbound) radiolabel."
• [Supplemental Figure 3] - "Specificity of the lamprey testis cytosolic moiety for various steroids including androstenedione (Ad), testosterone (T), 11-deoxycortisol (S), 5alpha-androstane-3,17-dione (5alpha-Ad), 5beta-androstane-3,17-dione (5beta-Ad), 5alpha-dihydrotestosterone (5alpha-DHT), and estrone (E₁)."