Supplemental Data

Files in this Data Supplement:

• [Supplemental Tables] - "SUPPLEMENTAL TABLE 1. Primers used for real-time PCR (qRT-PCR) of selected genes.

  SUPPLEMENTAL TABLE 2. Postnatal behavioral studies.

  SUPPLEMENTAL TABLE 3. Effect of ICSI with fresh or frozen sperm on organ to bodyweight ratio of 16-mo-old mice (CD1 mice)."

• [Supplemental Figure 1] - "Schematic representation of sequences analyzed by bisulphite sequencing of methylation profiles of two retransposable elements, intracisternal-A particle (Iap) and murine endogenous retrovirus-L (Erv4) and H19 gene. Circles represent CpG dinucleotides present in the regions analyzed. At the bottom is a design representing sequences upstream of the H19 promoter (arrow). The filled box on the line represents the 2 kb differentially methylated domain (DMD)."
• [Supplemental Figure 3] - "Free choice exploration paradigm in the Y maze of male and female CD1 mice produced by ICSI with frozen sperm. Animals were allowed to explore a three arm-maze, one of the arms unfamiliar (new) to the animals. Recognition of the novel arm is an index of spatial memory. The test was conducted using a short (2 min; A, B) or a long (30 min; C, D) intertrial interval. *P&[lt]0.01, novel versus familiar (Start or Neutral) arms of the maze."
• [Supplemental Figure 2] - "Performance in the elevated plus-maze (A, B) and in the open field (C, D) of 12- to 18-wk-old male and female CD1 mice produced by ICSI with frozen sperm. Animals were exposed on two consecutive days to the tests (first day is considered novelty, second day habituation). Parameters selected were indicative of locomotor activity (A, C) and exploration of aversive areas of the maze (B, D). Data are means &[plusmn] SEM of 8-10 determinations per group. *P &[lt]0.01, ICSI versus control animals. #P&[lt]0.01, habituation versus novelty conditions."