[Supplemental Figures] -
"Supplemental Figure 1. Immunohistochemistry for EFNB2, EPHB4 and LYVE1 in various tissues from B6 mice. Aadjacent sections were stained with EFNB2, EPHB4, or LYVE1 to identify arterial, venous, or lymphatic structures. Arterial endothelial cells of different tissue (arrowhead indicated) were clearly distinguished as EFNB2+ (row 2), EPHB4 (row 3). EPHB4 only marked the venous vessels (indicated as arrows). Lymphatic vessels were also clearly identified by LYVE1 (brown). A, artery; V, vein, B, bronchus or air passage in mice lung. Bar = 100 µm.
Supplemental Figure 2. Immunohistochemistry of EFNB2 (A) and EPHB4 (B) in virgin B6 estrus uterus. Boxed areas in A, B were shown to the right of each image at higher magnification. EFNB2 was detected on uterine artery endothelial cells discontinuously along with the vessel wall (A1). There was little EFNB2 expression in B6 uterine stroma but occasionally strong foci reactivity was found (arrowhead, A2). Weak expression was presented on uteri gland and epithelial cells facing the uterine lumen (arrow, A2). EPHB4 expression was weak and diffused in mesometrial endometrium but vessels were strongly stained (arrowheads, B1). Weak EPHB4 staining also occurred along the base of lumenal (arrow, B2) and glandular (arrowhead, B2) epithelial cells. AM, antimesometrial; G, uterine gland; L, lumen; M, mesometrial.
Supplemental Figure 3. Immunohistochemistry for LYVE1 in virgin uteri of B6 (A) and alymphoid (B) mice. Higher magnification images of boxed areas are to the right of each image. In both strains, most reactive endothelial structures were in the mesometrial triangle close to the myometrium and in endometrium. In alymphoid, some sporadic LYVE1 expression was detected on uterine lumen epithelium (arrowhead, B1) and reactive cells associated with uterine glands (arrowhead, B2). AM, antimesometrial; L, uterine lumen, M, mesometrial; G, uterine glands."
