| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Biology of Reproduction, Vol 55, 684-692, Copyright © 1996 by Society for the Study of Reproduction
ARTICLES |
P Leclerc, E de Lamirande and C Gagnon
Urology Research Laboratory, Royal Victoria Hospital, Montreal, Quebec, Canada.
The involvement of cAMP in the process of sperm capacitation has been the subject of several studies. In addition, the importance of protein- tyrosine phosphorylation in this process has been investigated, although only a few studies have been reported in the human. Since agents regulating the intracellular concentrations of cAMP affect sperm capacitation rates, the role of cAMP on the expression of phosphotyrosine-containing proteins was investigated during human sperm capacitation. Fetal cord serum ultrafiltrate, a known capacitation inducer in human spermatozoa, caused an increase in the phosphotyrosine content of 105- and 81-kDa proteins (p105 and p81), the two major phosphotyrosine-containing proteins of human spermatozoa. Similar effects were observed when spermatozoa were incubated with phosphodiesterase inhibitors or cell-permeant cAMP analogs, suggesting that cAMP is involved in these two processes. Forskolin, an adenylyl cyclase activator, also caused an increase in both sperm capacitation rates and tyrosine phosphorylation of p105 and p81, while 12-O- tetradecanoyl phorbol 13-acetate stimulated both capacitation and tyrosine phosphorylation of p105 and p81 only when spermatozoa were incubated in the presence of bicarbonate, in agreement with its reported effects on cAMP production and hamster sperm capacitation. The inhibition of these phenomena by cAMP-dependent protein kinase inhibitors, and the stimulation by protein phosphatase inhibitors, suggest that Ser/Thr protein phosphorylation plays an important role in the regulation of both sperm capacitation and protein-tyrosine phosphorylation pathways. However, observations that both calyculin A and okadaic acid stimulated sperm capacitation, whereas only calyculin A increased p105 and p81 phosphotyrosine content and sperm velocity, suggest that protein phosphatase PP1 is involved in the two latter phenomena while PP2A mediates sperm capacitation. These results suggest that divergent pathways might regulate tyrosine phosphorylation of p105 and p81 and sperm capacitation after cAMP-dependent phosphorylation of an intermediate protein.
This article has been cited by other articles:
|
|
 |
|
  S. Aquila, V. Rago, C. Guido, I. Casaburi, S. Zupo, and A. Carpino Leptin and leptin receptor in pig spermatozoa: evidence of their involvement in sperm capacitation and survival Reproduction, July 1, 2008; 136(1): 23 - 32. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Walsh, D. Whelan, A. Bielanowicz, B. Skinner, R. J. Aitken, M. K. O'Bryan, and B. Nixon Identification of the Molecular Chaperone, Heat Shock Protein 1 (Chaperonin 10), in the Reproductive Tract and in Capacitating Spermatozoa in the Male Mouse Biol Reprod, June 1, 2008; 78(6): 983 - 993. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Lachance, J. L. Bailey, and P. Leclerc Expression of Hsp60 and Grp78 in the human endometrium and oviduct, and their effect on sperm functions Hum. Reprod., October 1, 2007; 22(10): 2606 - 2614. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. S. Diaz, M. Kong, and P. Morales Effect of fibronectin on proteasome activity, acrosome reaction, tyrosine phosphorylation and intracellular calcium concentrations of human sperm Hum. Reprod., May 1, 2007; 22(5): 1420 - 1430. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Lefievre, K. Bedu-Addo, S. J Conner, G. S M Machado-Oliveira, Y. Chen, J. C Kirkman-Brown, M. A Afnan, S. J Publicover, W C. L Ford, and C. L R Barratt Counting sperm does not add up any more: time for a new equation? Reproduction, April 1, 2007; 133(4): 675 - 684. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A M Petrunkina, R A P Harrison, M Tsolova, E Jebe, and E Topfer-Petersen Signalling pathways involved in the control of sperm cell volume Reproduction, January 1, 2007; 133(1): 61 - 73. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.N. Jha, A.M. Salicioni, E. Arcelay, O. Chertihin, S. Kumari, J.C. Herr, and P.E. Visconti Evidence for the involvement of proline-directed serine/threonine phosphorylation in sperm capacitation Mol. Hum. Reprod., December 1, 2006; 12(12): 781 - 789. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Grasa, J. A. Cebrian-Perez, and T. Muino-Blanco Signal transduction mechanisms involved in in vitro ram sperm capacitation. Reproduction, November 1, 2006; 132(5): 721 - 732. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Baumber and S. A. Meyers Changes in Membrane Lipid Order With Capacitation in Rhesus Macaque (Macaca mulatta) Spermatozoa J Androl, July 1, 2006; 27(4): 578 - 587. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Baumber and S. A. Meyers Hyperactivated Motility in Rhesus Macaque (Macaca mulatta) Spermatozoa J Androl, May 1, 2006; 27(3): 459 - 468. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Baumber and S. A. Meyers Hyperactivated Motility in Rhesus Macaque (Macaca mulatta) Spermatozoa J Androl, May 1, 2006; 27(3): 459 - 468. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D.Y. Liu, G.N. Clarke, and H.W.G. Baker Tyrosine phosphorylation on capacitated human sperm tail detected by immunofluorescence correlates strongly with sperm-zona pellucida (ZP) binding but not with the ZP-induced acrosome reaction Hum. Reprod., April 1, 2006; 21(4): 1002 - 1008. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. F. Doncel Exploiting common targets in human fertilization and HIV infection: development of novel contraceptive microbicides Hum. Reprod. Update, March 1, 2006; 12(2): 103 - 117. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bajpai, S. E. Fiedler, Z. Huang, S. Vijayaraghavan, G. E. Olson, G. Livera, M. Conti, and D. W. Carr AKAP3 Selectively Binds PDE4A Isoforms in Bovine Spermatozoa Biol Reprod, January 1, 2006; 74(1): 109 - 118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Bedu-Addo, L. Lefievre, F.L.C. Moseley, C.L.R. Barratt, and S.J. Publicover Bicarbonate and bovine serum albumin reversibly 'switch' capacitation-induced events in human spermatozoa Mol. Hum. Reprod., September 1, 2005; 11(9): 683 - 691. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. C. N. Chiu, M.-K. Chung, H.-Y. Tsang, R. Koistinen, H. Koistinen, M. Seppala, K.-F. Lee, and W. S.B. Yeung Glycodelin-S in Human Seminal Plasma Reduces Cholesterol Efflux and Inhibits Capacitation of Spermatozoa J. Biol. Chem., July 8, 2005; 280(27): 25580 - 25589. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Dube, P. Leclerc, T. Baba, C. Reyes-Moreno, and J. L. Bailey The Proacrosin Binding Protein, sp32, Is Tyrosine Phosphorylated During Capacitation of Pig Sperm J Androl, July 1, 2005; 26(4): 519 - 528. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F.L.C. Moseley, K.N. Jha, L. Bjorndahl, I.A. Brewis, S.J. Publicover, C.L.R. Barratt, and L. Lefievre Protein tyrosine phosphorylation, hyperactivation and progesterone-induced acrosome reaction are enhanced in IVF media: an effect that is not associated with an increase in protein kinase A activation Mol. Hum. Reprod., July 1, 2005; 11(7): 523 - 529. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. O'Flaherty, E. de Lamirande, and C. Gagnon Reactive Oxygen Species and Protein Kinases Modulate the Level of Phospho-MEK-Like Proteins During Human Sperm Capacitation Biol Reprod, July 1, 2005; 73(1): 94 - 105. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G Buffone, J. C Calamera, S. V Verstraeten, and G. F Doncel Capacitation-associated protein tyrosine phosphorylation and membrane fluidity changes are impaired in the spermatozoa of asthenozoospermic patients Reproduction, June 1, 2005; 129(6): 697 - 705. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Liguori, E. de Lamirande, A. Minelli, and C. Gagnon Various protein kinases regulate human sperm acrosome reaction and the associated phosphorylation of Tyr residues and of the Thr-Glu-Tyr motif Mol. Hum. Reprod., March 1, 2005; 11(3): 211 - 221. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Laflamme, A. Akoum, and P. Leclerc Induction of human sperm capacitation and protein tyrosine phosphorylation by endometrial cells and interleukin-6 Mol. Hum. Reprod., February 1, 2005; 11(2): 141 - 150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Luconi, I. Porazzi, P. Ferruzzi, S. Marchiani, G. Forti, and E. Baldi Tyrosine Phosphorylation of the A Kinase Anchoring Protein 3 (AKAP3) and Soluble Adenylate Cyclase Are Involved in the Increase of Human Sperm Motility by Bicarbonate Biol Reprod, January 1, 2005; 72(1): 22 - 32. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. NagDas, V. P. Winfrey, and G. E. Olson Tyrosine Phosphorylation Generates Multiple Isoforms of the Mitochondrial Capsule Protein, Phospholipid Hydroperoxide Glutathione Peroxidase (PHGPx), During Hamster Sperm Capacitation Biol Reprod, January 1, 2005; 72(1): 164 - 171. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Nauc, E. De Lamirande, P. Leclerc, and C. Gagnon Inhibitors of Phosphoinositide 3-Kinase, LY294002 and Wortmannin, Affect Sperm Capacitation and Associated Phosphorylation of Proteins Differently: Ca2+-Dependent Divergences J Androl, July 1, 2004; 25(4): 573 - 585. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Willipinski-Stapelfeldt, J. Lubberstedt, S. Stelter, K. Vogt, A. K. Mukhopadhyay, and D. Muller Comparative analysis between cyclic GMP and cyclic AMP signalling in human sperm Mol. Hum. Reprod., July 1, 2004; 10(7): 543 - 552. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Marquez and S. S. Suarez Different Signaling Pathways in Bovine Sperm Regulate Capacitationand Hyperactivation Biol Reprod, June 1, 2004; 70(6): 1626 - 1633. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R E Spindler, Y Huang, J G Howard, P Wang, H Zhang, G Zhang, and D E Wildt Acrosomal integrity and capacitation are not influenced by sperm cryopreservation in the giant panda Reproduction, May 1, 2004; 127(5): 547 - 556. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. O'Flaherty, E. de Lamirande, and C. Gagnon Phosphorylation of the Arginine-X-X-(Serine/Threonine) motif in human sperm proteins during capacitation: modulation and protein kinase A dependency Mol. Hum. Reprod., May 1, 2004; 10(5): 355 - 363. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Luconi, V. Carloni, F. Marra, P. Ferruzzi, G. Forti, and E. Baldi Increased phosphorylation of AKAP by inhibition of phosphatidylinositol 3-kinase enhances human sperm motility through tail recruitment of protein kinase A J. Cell Sci., March 1, 2004; 117(7): 1235 - 1246. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Rivlin, J. Mendel, S. Rubinstein, N. Etkovitz, and H. Breitbart Role of Hydrogen Peroxide in Sperm Capacitation and Acrosome Reaction Biol Reprod, February 1, 2004; 70(2): 518 - 522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Baker, L. Hetherington, H. Ecroyd, S. D. Roman, and R. J. Aitken Analysis of the mechanism by which calcium negatively regulates the tyrosine phosphorylation cascade associated with sperm capacitation J. Cell Sci., January 15, 2004; 117(2): 211 - 222. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.G. Buffone, G.F. Doncel, C.I. Marin Briggiler, M.H. Vazquez-Levin, and J.C. Calamera Human sperm subpopulations: relationship between functional quality and protein tyrosine phosphorylation Hum. Reprod., January 1, 2004; 19(1): 139 - 146. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Turner Tales From the Tail: What Do We Really Know About Sperm Motility? J Androl, November 1, 2003; 24(6): 790 - 803. [Full Text] [PDF]
|
|
|
|
|
|
N. L. Cross Decrease in Order of Human Sperm Lipids During Capacitation Biol Reprod, August 1, 2003; 69(2): 529 - 534. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Cormier and J. L. Bailey A Differential Mechanism Is Involved During Heparin- and Cryopreservation-Induced Capacitation of Bovine Spermatozoa Biol Reprod, July 1, 2003; 69(1): 177 - 185. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. W. Ecroyd, R. C. Jones, and R. J. Aitken Endogenous Redox Activity in Mouse Spermatozoa and Its Role in Regulating the Tyrosine Phosphorylation Events Associated with Sperm Capacitation Biol Reprod, July 1, 2003; 69(1): 347 - 354. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.J. de Vries, T. Wiedmer, P.J. Sims, and B.M. Gadella Caspase-Independent Exposure of Aminophospholipids and Tyrosine Phosphorylation in Bicarbonate Responsive Human Sperm Cells Biol Reprod, June 1, 2003; 68(6): 2122 - 2134. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-T. Zeng and D. R. P. Tulsiani Calmodulin antagonists differentially affect capacitation-associated protein tyrosine phosphorylation of mouse sperm components J. Cell Sci., May 15, 2003; 116(10): 1981 - 1989. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Thundathil, E. de Lamirande, and C. Gagnon Nitric Oxide Regulates the Phosphorylation of the Threonine-Glutamine-Tyrosine Motif in Proteins of Human Spermatozoa During Capacitation Biol Reprod, April 1, 2003; 68(4): 1291 - 1298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Nimmo and N. L. Cross Structural Features of Sterols Required to Inhibit Human Sperm Capacitation Biol Reprod, April 1, 2003; 68(4): 1308 - 1317. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Pommer, J. Rutllant, and S. A. Meyers Phosphorylation of Protein Tyrosine Residues in Fresh and Cryopreserved Stallion Spermatozoa under Capacitating Conditions Biol Reprod, April 1, 2003; 68(4): 1208 - 1214. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ficarro, O. Chertihin, V. A. Westbrook, F. White, F. Jayes, P. Kalab, J. A. Marto, J. Shabanowitz, J. C. Herr, D. F. Hunt, et al. Phosphoproteome Analysis of Capacitated Human Sperm. EVIDENCE OF TYROSINE PHOSPHORYLATION OF A KINASE-ANCHORING PROTEIN 3 AND VALOSIN-CONTAINING PROTEIN/p97 DURING CAPACITATION J. Biol. Chem., March 21, 2003; 278(13): 11579 - 11589. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Dorval, M. Dufour, and P. Leclerc Role of protein tyrosine phosphorylation in the thapsigargin-induced intracellular Ca2+ store depletion during human sperm acrosome reaction Mol. Hum. Reprod., March 1, 2003; 9(3): 125 - 131. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Fujinoki, T. Kawamura, T. Toda, H. Ohtake, T. Ishimoda-Takagi, N. Shimizu, S. Yamaoka, and M. Okuno Identification of 36-kDa Flagellar Phosphoproteins Associated with Hamster Sperm Motility J. Biochem., March 1, 2003; 133(3): 361 - 369. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Fournier, P. Leclerc, N. Cormier, and J. L. Bailey Implication of Calmodulin-Dependent Phosphodiesterase Type 1 During Bovine Sperm Capacitation J Androl, January 1, 2003; 24(1): 104 - 112. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tardif, C. Dube, and J. L. Bailey Porcine Sperm Capacitation and Tyrosine Kinase Activity Are Dependent on Bicarbonate and Calcium but Protein Tyrosine Phosphorylation Is Only Associated with Calcium Biol Reprod, January 1, 2003; 68(1): 207 - 213. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Furusawa, T. Taira, S. M. M. Iguchi-Ariga, and H. Ariga AMY-1 Interacts with S-AKAP84 and AKAP95 in the Cytoplasm and the Nucleus, Respectively, and Inhibits cAMP-dependent Protein Kinase Activity by Preventing Binding of Its Catalytic Subunit to A-kinase-anchoring Protein (AKAP) Complex J. Biol. Chem., December 20, 2002; 277(52): 50885 - 50892. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Yukitake, M. Furusawa, T. Taira, S. M. M. Iguchi-Ariga, and H. Ariga AAT-1, a Novel Testis-specific AMY-1-binding Protein, Forms a Quaternary Complex with AMY-1, A-kinase Anchor Protein 84, and a Regulatory Subunit of cAMP-dependent Protein Kinase and Is Phosphorylated by Its Kinase J. Biol. Chem., November 15, 2002; 277(47): 45480 - 45492. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Dorval, M. Dufour, and P. Leclerc Regulation of the Phosphotyrosine Content of Human Sperm Proteins by Intracellular Ca2+: Role of Ca2+-Adenosine Triphosphatases Biol Reprod, November 1, 2002; 67(5): 1538 - 1545. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Thundathil, E. de Lamirande, and C. Gagnon Different signal transduction pathways are involved during human sperm capacitation induced by biological and pharmacological agents Mol. Hum. Reprod., September 1, 2002; 8(9): 811 - 816. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Lefievre, K. N. Jha, E. de Lamirande, P. E. Visconti, and C. Gagnon Activation of Protein Kinase A During Human Sperm Capacitation and Acrosome Reaction J Androl, September 1, 2002; 23(5): 709 - 716. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Lefievre, E. de Lamirande, and C. Gagnon Presence of Cyclic Nucleotide Phosphodiesterases PDE1A, Existing as a Stable Complex with Calmodulin, and PDE3A in Human Spermatozoa Biol Reprod, August 1, 2002; 67(2): 423 - 430. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. V. Holt and R. A. P. Harrison Bicarbonate Stimulation of Boar Sperm Motility via a Protein Kinase A--Dependent Pathway: Between-Cell and Between-Ejaculate Differences Are Not Due to Deficiencies in Protein Kinase A Activation J Androl, July 1, 2002; 23(4): 557 - 565. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Leclerc and S. Goupil Regulation of the Human Sperm Tyrosine Kinase c-yes. Activation by Cyclic Adenosine 3',5'-Monophosphate and Inhibition by Ca2+ Biol Reprod, July 1, 2002; 67(1): 301 - 307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.C. Kirkman-Brown, L. Lefievre, C. Bray, P.M. Stewart, C.L.R. Barratt, and S.J. Publicover Inhibitors of receptor tyrosine kinases do not suppress progesterone-induced [Ca2+]i signalling in human spermatozoa Mol. Hum. Reprod., April 1, 2002; 8(4): 326 - 332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. de Lamirande and C. Gagnon The extracellular signal-regulated kinase (ERK) pathway is involved in human sperm function and modulated by the superoxide anion Mol. Hum. Reprod., February 1, 2002; 8(2): 124 - 135. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. Herrero, E. de Lamirande, and C. Gagnon Tyrosine nitration in human spermatozoa: a physiological function of peroxynitrite, the reaction product of nitric oxide and superoxide Mol. Hum. Reprod., October 1, 2001; 7(10): 913 - 921. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tardif, C. Dube, S. Chevalier, and J. L. Bailey Capacitation Is Associated with Tyrosine Phosphorylation and Tyrosine Kinase-Like Activity of Pig Sperm Proteins Biol Reprod, September 1, 2001; 65(3): 784 - 792. [Abstract] [Full Text] [PDF]
|
|
