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Molecular Characterization of Heat Shock-Like Factor Encoded on the Human Y Chromosome, and Implications for Male Infertility¹

Department of Human Genetics and Public Health,³ Graduate School of Proteomics, Faculty of Medicine, The University of Tokushima, Tokushima-City, 770-8503 Japan Department of Urology,⁴ St. Marianna Medical University, School of Medicine, Kawasaki, 216-8511 Japan Departments of Urology⁵ Anatomy and Cell Biology,⁶ School of Medicine, University of Tokushima, Tokushima-City, 770-8503 Japan Core Research for Evolutional Science and Technology Corporation,⁷ Saitama, 332-0012 Japan

Azoospermia and oligospermia are major causes of male infertility. Some genes located on the Y chromosome are suggested as candidates. Recently, HSFY, which is similar to the HSF (heat shock transcription factor) family, has been mapped on the human Y chromosome as multicopies. However, newly available sequence data deposited at NCBI shows that only the HSFY gene located on Yq has a long open reading frame containing a HSF-type DNA-binding domain. HSFY is similar to LW-1 on the human X chromosome and a murine HSFY-like sequence (mHSFYL), 4933413G11Rik, on the mouse chromosome 1. LW-1 and mHSFYL have 53% and 70% homology to HSFY for amino acid sequences of their presumed DNA-binding domains, respectively. Comparison of the presumed DNA-binding domains unveiled that the three HSF-like factors, HSFY, LW-1, and mHSFYL, belong to a different class than conventional HSFs. When we screened for deletions on the Yq of males suffering from infertility, we found that HSFY was involved in interstitial deletions on the Y chromosomes for two azoospermic males who had DBY, USP9Y, and DAZ but did not have RBMY located on the AZFb. Expression analysis of HSFY, LW-1, and mHSFYL unveiled that they are expressed predominantly in testis. Furthermore, immunhistochemistry of HSFY in testis showed that its expression is restricted to both Sertoli cells and spermatogenic cells and that it exhibits a stage-dependent translocation from the cytoplasm to the nucleus in spermatogenetic cells during spermatogenesis. These results may suggest that deletion of HSFY is involved in azoospermia or oligospermia.

¹ Supported in part by grants from the Core Research for Evolutional Science and Technology and Ministry of Health, Labour, and Welfare, Japan.

² Correspondence: Yutaka Nakahori, Department of Human Genetics and Public Health, School of Medicine, The University of Tokushima, 3-18-15 Kuramoto-cho, Tokushima-City, 770-8503 Japan. FAX: 81 88 633 7453; nakahori{at}basic.med.tokushima-u.ac.jp

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