| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Research Article |
Developmental Endocrinology and Endocrine Disruptor Section,3 Laboratory of Molecular Toxicology, National Institute of Environmental Health Sciences, NIH/DHHS, Research Triangle Park, North Carolina 27709
Department of Environmental and Molecular Toxicology,4 North Carolina State University, Raleigh, North Carolina 27605
Department of Biology,5 Syracuse University, Syracuse, New York 13210
ABSTRACT
Early in ovarian differentiation, female mouse germ cells develop in clusters called oocyte nests or germline cysts. After birth, mouse germ cell nests break down into individual oocytes that are surrounded by somatic pregranulosa cells to form primordial follicles. Previously, we have shown that mice treated neonatally with genistein, the primary soy phytoestrogen, have multi-oocyte follicles (MOFs), an effect apparently mediated by estrogen receptor 2 (ESR2, more commonly known as ERbeta). To determine if genistein treatment leads to MOFs by inhibiting breakdown of oocyte nests, mice were treated neonatally with genistein (50 mg/kg per day) on Days 1–5, and the differentiation of the ovary was compared with untreated controls. Mice treated with genistein had fewer single oocytes and a higher percentage of oocytes not enclosed in follicles. Oocytes from genistein-treated mice exhibited intercellular bridges at 4 days of age, long after disappearing in controls by 2 days of age. There was also an increase in the number of oocytes that survived during the nest breakdown period and fewer oocytes undergoing apoptosis on Neonatal Day 3 in genistein-treated mice as determined by poly (ADP-ribose) polymerase (PARP1) and deoxynucleotidyl transferase mediated deoxyuridine triphosphate nick end-labeling (TUNEL). These data taken together suggest that genistein exposure during development alters ovarian differentiation by inhibiting oocyte nest breakdown and attenuating oocyte cell death.
developmental biology, environment, mechanisms of hormone action, ovary
1 This research was supported by the Intramural Research Program of the NIH, National Institute of Environmental Health Sciences.
2 Correspondence: Wendy Jefferson, NIEHS, 111 Alexander Dr., Research Triangle Park, NC 27709. FAX: 919 541 4634; jeffers1{at}niehs.nih.gov
This article has been cited by other articles:
|
|
 |
|
  S. A. Williams and P. Stanley Mouse fertility is enhanced by oocyte-specific loss of core 1-derived O-glycans FASEB J, July 1, 2008; 22(7): 2273 - 2284. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.Y. Yang and J.E. Fortune The Capacity of Primordial Follicles in Fetal Bovine Ovaries to Initiate Growth In Vitro Develops During Mid-Gestation and Is Associated with Meiotic Arrest of Oocytes Biol Reprod, June 1, 2008; 78(6): 1153 - 1161. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. Fowler, N. J. Dora, H. McFerran, M. R. Amezaga, D. W. Miller, R. G. Lea, P. Cash, A. S. McNeilly, N. P. Evans, C. Cotinot, et al. In utero exposure to low doses of environmental pollutants disrupts fetal ovarian development in sheep Mol. Hum. Reprod., May 1, 2008; 14(5): 269 - 280. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Sotomayor-Zarate, M. Dorfman, A. Paredes, and H. E. Lara Neonatal Exposure to Estradiol Valerate Programs Ovarian Sympathetic Innervation and Follicular Development in the Adult Rat Biol Reprod, April 1, 2008; 78(4): 673 - 680. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Mayo, L. Jameson, and T. K. Woodruff Eggs in the Nest Endocrinology, August 1, 2007; 148(8): 3577 - 3579. [Full Text] [PDF]
|
|
|
|
|
|
Y. Chen, W. N. Jefferson, R. R. Newbold, E. Padilla-Banks, and M. E. Pepling Estradiol, Progesterone, and Genistein Inhibit Oocyte Nest Breakdown and Primordial Follicle Assembly in the Neonatal Mouse Ovary in Vitro and in Vivo Endocrinology, August 1, 2007; 148(8): 3580 - 3590. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M Dorward, K. L Shultz, and W. G Beamer LH analog and dietary isoflavones support ovarian granulosa cell tumor development in a spontaneous mouse model Endocr. Relat. Cancer, June 1, 2007; 14(2): 369 - 379. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. B John, L. J Shirley, T. D Gallardo, and D. H Castrillon Specificity of the requirement for Foxo3 in primordial follicle activation Reproduction, May 1, 2007; 133(5): 855 - 863. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Kipp, S. M. Kilen, S. Bristol-Gould, T. K. Woodruff, and K. E. Mayo Neonatal Exposure to Estrogens Suppresses Activin Expression and Signaling in the Mouse Ovary Endocrinology, May 1, 2007; 148(5): 1968 - 1976. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. E Nilsson, J. Stanfield, and M. K Skinner Interactions between progesterone and tumor necrosis factor-{alpha} in the regulation of primordial follicle assembly. Reproduction, December 1, 2006; 132(6): 877 - 886. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Padilla-Banks, W. N. Jefferson, and R. R. Newbold Neonatal Exposure to the Phytoestrogen Genistein Alters Mammary Gland Growth and Developmental Programming of Hormone Receptor Levels Endocrinology, October 1, 2006; 147(10): 4871 - 4882. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
