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PSPC1, NONO, and SFPQ Are Expressed in Mouse Sertoli Cells and May Function as Coregulators of Androgen Receptor-Mediated Transcription¹

Department of Environment and Natural Sciences,³ Graduate School of Environment and Information Sciences, Yokohama National University, Yokohama 240-8501, Japan Division of Molecular Genetics Research,⁴ Life Science Research Center, University of Toyama, Toyama 930-0194, Japan Department of Developmental Biology,⁵ Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima 734-8553, Japan Department of Cell Biology,⁶ Institute of Development, Aging and Cancer, Tohoku University, Sendai, 980-8575, Japan

ABSTRACT

In Sertoli cells of testis, androgen receptor-regulated gene transcription plays an indispensable role in maintaining spermatogenesis. Androgen receptor activity is modulated by a number of coregulators which are associated with the androgen receptor. Non-POU-domain-containing, octamer binding protein (NONO), a member of the DBHS-containing proteins, complexes with androgen receptor and functions as a coactivator for the receptor. Paraspeckle protein 1 alpha isoform (PSPC1, previously known as PSP1) and Splicing factor, proline- and glutamine-rich (SFPQ, previously known as PSF), other members of the DBHS-containing proteins, are also found in androgen receptor complexes, suggesting that these DBHS-containing proteins may cooperatively regulate androgen receptor-mediated gene transcription. We demonstrated that PSPC1, NONO, and SFPQ are coexpressed in Sertoli cell line TTE3 and interact reciprocally. The effect of the DBHS-containing proteins on the transcriptional activity was assessed using the construct containing androgen-responsive elements followed by a luciferase gene. The results showed that all the DBHS-containing proteins activate androgen receptor-mediated transcription, and PSPC1 is the most effective coactivator among them. Furthermore, we confirmed the presence of PSPC1, NONO, and SFPQ proteins in Sertoli cells of adult mouse testis sections. These observations suggest that PSPC1, NONO, and SFPQ form complexes with each other in Sertoli cells and may regulate androgen receptor-mediated transcriptional activity.

androgen receptor, gene regulation, Sertoli cells, spermatogenesis, testis

¹ Supported by grants from the Ministry of Education, Sports, Culture, Science and Technology of Japan (MEXT) to Y.K.

² Correspondence: Yasuyuki Kurihara, Department of Environment and Natural Sciences, Graduate School of Environment and Information Sciences, Yokohama National University, Tokiwa-dai, Hodogaya, Yokohama 240-8501, Japan. FAX: 81 45 339 4263; kurihara{at}ynu.ac.jp

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