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This Article Full Text Full Text (PDF) All Versions of this Article: 76/6/1002    most recent biolreprod.106.059881v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yamauchi, Y. Articles by Ward, M. A. Search for Related Content PubMed PubMed Citation Articles by Yamauchi, Y. Articles by Ward, M. A. Agricola Articles by Yamauchi, Y. Articles by Ward, M. A.

Preservation of Ejaculated Mouse Spermatozoa from Fertile C57BL/6 and Infertile Hook1/Hook1 Mice Collected from the Uteri of Mated Females¹

Institute for Biogenesis Research, University of Hawaii Medical School, Honolulu, Hawaii 96822

ABSTRACT

Methods routinely used to preserve mouse spermatozoa require that the male be killed to recover spermatozoa from the epididymides. Here we obtained multiple samples of ejaculated spermatozoa from normal fertile C57BL/6 and infertile Hook1/Hook1 (formerly known as azh/azh) mutant males from uteri after mating, thus avoiding termination of the males. Ejaculated sperm were preserved by conventional cryopreservation or by rapid freezing without cryoprotection, and were injected into the oocytes by intracytoplasmic sperm injection (ICSI). The proportions of oocytes that survived, became activated, and developed into two-cell embryos were similar when comparing the two preservation methods in wild-type versus Hook1/Hook1 mice and tested mice versus controls (fresh and rapid-frozen epididymal and fresh ejaculated sperm). Two-cell embryos were transferred into the oviducts of pseudopregnant females, and fetal development was examined at Day 15 of gestation. A total of 39%–54% of transferred embryos produced with preserved ejaculated sperm implanted. Live, normal fetuses (11%–17%) were obtained in all examined groups and from all males included in the study. More implants (71%–82%) and fetuses (28%–31%) were noted in controls. Lower developmental potentials of embryos produced with preserved ejaculated sperm might be due to their capacitation status; the majority of sperm retrieved from the uterus were capacitated. This study bears significance for the maintenance and distribution of novel mouse strains. The method is applicable for all types of mice, including those with male infertility syndromes. The sole requirement is that the male of interest is able to copulate and its ejaculate contains spermatozoa.

assisted reproductive technology,, embryo, gamete biology, in vitro fertilization, sperm

¹Supported by National Institutes of Health grant HD048446 to M.A.W.

Correspondence: ²Monika A. Ward, Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii, 1960 East-West Rd., Honolulu, HI 96822. FAX: 808 956 7316; e-mail: mward{at}hawaii.edu

This article has been cited by other articles:

				Y. Yamauchi, A. Ajduk, J. M Riel, and M. A Ward Ejaculated and Epididymal Mouse Spermatozoa Are Different in Their Susceptibility to Nuclease-Dependent DNA Damage and in Their Nuclease Activity Biol Reprod, October 1, 2007; 77(4): 636 - 647. [Abstract] [Full Text] [PDF]