Biol Reprod Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

BOR - Papers in Press, published online ahead of print February 6, 2008.
Biol Reprod 2008, 10.1095/biolreprod.107.067033
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
78/5/896    most recent
biolreprod.107.067033v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yan, W.
Right arrow Articles by Yanagimachi, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yan, W.
Right arrow Articles by Yanagimachi, R.
Agricola
Right arrow Articles by Yan, W.
Right arrow Articles by Yanagimachi, R.
BIOLOGY OF REPRODUCTION 78, 896–902 (2008)
DOI: 10.1095/biolreprod.107.067033
© 2008 by the Society for the Study of Reproduction, Inc.

research-article

Birth of Mice after Intracytoplasmic Injection of Single Purified Sperm Nuclei and Detection of Messenger RNAs and MicroRNAs in the Sperm Nuclei1

Wei Yan 2 4, Kazuto Morozumi 5, Jie Zhang 4, Seungil Ro 4, Chanjae Park 4, and Ryuzo Yanagimachi 3 5

Department of Physiology and Cell Biology,4 University of Nevada School of Medicine, Reno, Nevada 89557 Institute for Biogenesis Research,5 University of Hawaii School of Medicine, Honolulu, Hawaii 96822

ABSTRACT

We have developed a method that effectively removes all of the perinuclear materials of a mouse sperm head, including the acrosome, plasma membrane, perinuclear theca, and nuclear envelope. By injection of a single purified sperm head into a metaphase II mouse oocyte followed by activation with strontium chloride, 93% of the zygotes developed into two-cell embryos. Although only ~17% of the transferred two-cell embryos were born alive, all live pups developed into adults, and they appeared to be normal in reproduction and behavior. We detected RNA species, including mRNAs and miRNAs from the purified sperm heads. Our data demonstrate that pure membrane-free sperm heads are sufficient to produce normal offspring through intracytoplasmic sperm injection and that at least part of the RNA molecules are deeply embedded in the sperm nucleus.

assisted reproductive technology, early development, embryo, fertility, fertilization, intracytoplasmic sperm injection, in vitro fertilization, small RNAs, sperm

FOOTNOTES

1Supported by a start-up fund from the University of Nevada Reno to W.Y.

Correspondence: 2Wei Yan, Department of Physiology and Cell Biology, University of Nevada School of Medicine, Anderson Biomedical Science Bldg. 105C/111, 1664 North Virginia St., MS 352, Reno, NV 89557. FAX: 775 784 6903; e-mail: wyan{at}medicine.nevada.edu

Correspondence: 3Ryuzo Yanagimachi, Institute for Biogenesis Research, University of Hawaii School of Medicine, 1960 East-West Rd., Honolulu, HI 96822. FAX: 808 956 7316; e-mail: yana{at}hawaii.edu






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2008 by the Society for the Study of Reproduction.