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BOR - Papers in Press, published online ahead of print October 17, 2002.
Biol Reprod 2002, 10.1095/biolreprod.102.005140
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Submitted March 5, 2002
Returned for revision March 25, 2002
Accepted July 16, 2002

Testis


Mitochondrial DNA Content of Human Spermatozoa

Carmen Díez-Sánchez 1, Eduardo Ruiz-Pesini 1, Ana Cristina Lapeña 1, Julio Montoya 1, Acisclo Pérez-Martos 1, José Antonio Enriquez 1, Manuel J. López-Pérez 2*
1 Universidad de Zaragoza
2 University of Zaragoza

* To whom correspondence should be addressed. E-mail: lopezper{at}posta.unizar.es.

Abstract

Sperm mitochondria play an important role in spermatozoa due to the high ATP demand of these cells. In agreement with that, it has been reported that different mitochondrial DNA mutations and haplogroups have influence in sperm function. The mitochondrial DNA (mtDNA) dose also contributes to genetic variability and pathology in different tissues and organs but nothing is known about its relevance in the performance of spermatozoa. In the present report, we have estimated the variability in mtDNA content within a population of men. Thus, different mtDNA/nDNA ratios were found to be characteristic of progressive and non-progressive spermatozoa, confirming the influence of mtDNA content in sperm functionality. In addition, we have been able to estimate that the absolute content of mtDNA was 700 and 1,200 mtDNA copies per cell in progressive and non-progressive human spermatozoa, respectively. These results suggest that a remarkable increase of mtDNA copy number per cell volume takes place during spermatogenesis.



Key words: Gamete Biology • Sperm • Sperm maturation • Spermatogenesis


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