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BOR - Papers in Press, published online ahead of print October 17, 2002.
Biol Reprod 2002, 10.1095/biolreprod.102.007906
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Submitted May 29, 2002
Returned for revision June 13, 2002
Accepted September 5, 2002

Embryo


MuERV-L Is One of the Earliest Transcribed Genes in Mouse One-Cell Embryos

Daisuke Kigami 1, Naojiro Minami 1*, Hanae Takayama 1, Hiroshi Imai 1
1 Kyoto University

* To whom correspondence should be addressed. E-mail: naojiro{at}kais.kyoto-u.ac.jp.

Abstract

The expression pattern and function of the MuERV-L gene in mouse preimplantation embryos was investigated. MuERV-L was rapidly transcribed from the beginning of the S phase (8 h after fertilization) of the first cell cycle. MuERV-L expression was completely repressed when transcription from the zygotic genome was inhibited by {alpha}-amanitin. These results reveal that MuERV-L is transcribed from the zygotic genome and that it is expressed earlier than any other genes previously reported. In addition, MuERV-L was expressed even when the first round of DNA synthesis was inhibited by aphidicolin, suggesting that expression of the gene is controlled by the zygotic clock. The function of MuERV-L in the development of mouse embryos was also examined using antisense oligonucleotides. The developmental competence of embryos was markedly suppressed after the 4-cell stage when the embryos were treated with antisense oligonucleotides. This result suggests that MuERV-L plays an important role in the development of mouse embryos at the early preimplantation stage.



Key words: Embryo • Developmental biology • Early development • Gene regulation


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