Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

BOR - Papers in Press, published online ahead of print October 17, 2002.
Biol Reprod 2002, 10.1095/biolreprod.102.008334
This Article
Right arrow Full Text (Rapid PDF)
Right arrow All Versions of this Article:
68/2/604    most recent
biolreprod.102.008334v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Author home page(s):
Satoshi Ohashi
Kunihiko Naito
Koji Sugiura
Naoki Iwamori
Seitaro Goto
Haruna Naruoka
Hireaki Tojo
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ohashi, S.
Right arrow Articles by Tojo, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ohashi, S.
Right arrow Articles by Tojo, H.
Agricola
Right arrow Articles by Ohashi, S.
Right arrow Articles by Tojo, H.
Submitted June 17, 2002
Returned for revision July 11, 2002
Accepted September 3, 2002

Gamete Biology


Analyses of Mitogen-Activated Protein Kinase Function in the Maturation of Porcine Oocytes

Satoshi Ohashi 1, Kunihiko Naito 2*, Koji Sugiura 1, Naoki Iwamori 1, Seitaro Goto 1, Haruna Naruoka 1, Hireaki Tojo 1
1 Laboratory of Applied Genetics, Department of Animal Resourc
2 University of Tokyo

* To whom correspondence should be addressed. E-mail: aknaito{at}mail.ecc.u-tokyo.ac.jp.

Abstract

The function of mitogen-activated protein kinase (MAPK) during porcine oocyte maturation was examined by injecting oocytes with either mRNA or antisense RNA of porcine c-mos protein, an upstream kinase of MAPK. The RNAs were injected into the cytoplasm of porcine immature oocytes immediately after collection from ovaries, then the oocytes were cultured for maturation up to 48h. The phosphorylation and activation of MAPK were observed at 6h after injection of the c-mos mRNA injected-oocytes, whereas in control oocytes, MAPK activation was detected at 24h of culture. The GVBD rate at 24h of culture was significantly higher in c-mos mRNA-injected oocytes than in control oocytes. In contrast, although injection of c-mos antisense RNA completely inhibited phosphorylation and activation of MAPK throughout the maturation period, the GVBD rate and its time course were the same in non-injected oocytes. The degree of maturation promoting factor (MPF) activation was, however, very low in oocytes in the absence of MAPK activation. Most of those oocytes had both abnormal morphology and decondensed chromosomes at 48 h of culture. These results suggest that MAPK activation is not required for GVBD induction in porcine oocytes, and that the major roles of MAPK during porcine oocyte maturation are to promote GVBD by increasing MPF activity and to arrest the oocytes at the second metaphase.



Key words: Kinases • Meiosis • Oocyte development • Ovum • Signal transduction


This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
M. Li, J.-S. Ai, B.-Z. Xu, B. Xiong, S. Yin, S.-L. Lin, Y. Hou, D.-Y. Chen, H. Schatten, and Q.-Y. Sun
Testosterone Potentially Triggers Meiotic Resumption by Activation of Intra-Oocyte SRC and MAPK in Porcine Oocytes
Biol Reprod, November 1, 2008; 79(5): 897 - 905.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. Cecconi, A. Mauro, G. Capacchietti, P. Berardinelli, N. Bernabo, A. R. Di Vincenzo, M. Mattioli, and B. Barboni
Meiotic Maturation of Incompetent Prepubertal Sheep Oocytes Is Induced by Paracrine Factor(s) Released by Gonadotropin-Stimulated Oocyte-Cumulus Cell Complexes and Involves Mitogen-Activated Protein Kinase Activation
Endocrinology, January 1, 2008; 149(1): 100 - 107.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
C.-G. Liang, Y.-Q. Su, H.-Y. Fan, H. Schatten, and Q.-Y. Sun
Mechanisms Regulating Oocyte Meiotic Resumption: Roles of Mitogen-Activated Protein Kinase
Mol. Endocrinol., September 1, 2007; 21(9): 2037 - 2055.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
S. Kume, T. Endo, Y. Nishimura, K. Kano, and K. Naito
Porcine SPDYA2 (RINGO A2) Stimulates CDC2 Activity and Accelerates Meiotic Maturation of Porcine Oocytes
Biol Reprod, March 1, 2007; 76(3): 440 - 447.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
K. Nganvongpanit, H. Muller, F. Rings, M. Hoelker, D. Jennen, E. Tholen, V. Havlicek, U. Besenfelder, K. Schellander, and D. Tesfaye
Selective degradation of maternal and embryonic transcripts in in vitro produced bovine oocytes and embryos using sequence specific double-stranded RNA.
Reproduction, May 1, 2006; 131(5): 861 - 874.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
K. Sugiura, K. Naito, T. Endo, and H. Tojo
Study of Germinal Vesicle Requirement for the Normal Kinetics of Maturation/M-Phase-Promoting Factor Activity During Porcine Oocyte Maturation
Biol Reprod, March 1, 2006; 74(3): 593 - 600.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. Craig, H. Zhu, P. W. Dyce, J. Petrik, and J. Li
Leptin Enhances Oocyte Nuclear and Cytoplasmic Maturation via the Mitogen-Activated Protein Kinase Pathway
Endocrinology, November 1, 2004; 145(11): 5355 - 5363.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
L.-J. Huo, H.-Y. Fan, C.-G. Liang, L.-Z. Yu, Z.-S. Zhong, D.-Y. Chen, and Q.-Y. Sun
Regulation of Ubiquitin-Proteasome Pathway on Pig Oocyte Meiotic Maturation and Fertilization
Biol Reprod, September 1, 2004; 71(3): 853 - 862.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
H.-Y. Fan, L.-J. Huo, D.-Y. Chen, H. Schatten, and Q.-Y. Sun
Protein Kinase C and Mitogen-Activated Protein Kinase Cascade in Mouse Cumulus Cells: Cross Talk and Effect on Meiotic Resumption of Oocyte
Biol Reprod, April 1, 2004; 70(4): 1178 - 1187.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
H.-Y. Fan and Q.-Y. Sun
Involvement of Mitogen-Activated Protein Kinase Cascade During Oocyte Maturation and Fertilization in Mammals
Biol Reprod, March 1, 2004; 70(3): 535 - 547.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
T. Kuroda, K. Naito, K. Sugiura, M. Yamashita, I. Takakura, and H. Tojo
Analysis of the Roles of Cyclin B1 and Cyclin B2 in Porcine Oocyte Maturation by Inhibiting Synthesis with Antisense RNA Injection
Biol Reprod, January 1, 2004; 70(1): 154 - 159.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2002 by the Society for the Study of Reproduction.