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BOR - Papers in Press, published online ahead of print December 11, 2002.
Biol Reprod 2002, 10.1095/biolreprod.102.008367
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Submitted June 13, 2002
Returned for revision July 5, 2002
Accepted September 24, 2002

Female Reproductive Tract


Contraction of Cultured Human Uterine Smooth Muscle Cells after Stimulation with Endothelin-1

Emmanuelle Dallot , Marcel Pouchelet , Nelly Gouhier , Dominique Cabrol , Françoise Ferré , Michelle Breuiller-Fouché *

* To whom correspondence should be addressed. E-mail: breuiller-fouche{at}cochin.inserm.fr.

Abstract

The problem of how to maintain isolated smooth cells in a "contractile" phenotypic state without deviation after subculturing has yet to be resolved. The present study characterized the in vitro contractile response of human uterine smooth muscle cell to endothelin-1, which is known to induce contractions in isolated uterine strips. Contractile effects were qualitatively investigated using silicone rubber substrata. Endothelin-1 was able to distort and reduce the wrinkles in the silicone surface. Contractions were also quantified by measuring the resulting change in the collagen lattice area. Endothelin-1 significantly increased the contractile response in a dose-dependent manner by selectively activating endothelin A receptors. When myometrial cells were cultured within collagen lattices, a microfilament-disrupting agent, cytochalasin B, abolished contractions and there was no change in alpha-smooth muscle actin immunostaining. Taken together, these observations show that the uterine smooth muscle cells are contractile and respond appropriately to a potent uterotonic agent. These findings proposed a cultured uterine smooth muscle cell model, which could be used to elucidate the mechanisms controlling uterine activity.



Key words: Mechanisms of Hormone Action • Parturition • Signal transduction • Uterus


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