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BOR - Papers in Press, published online ahead of print October 14, 2002.
Biol Reprod 2002, 10.1095/biolreprod.102.008706
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Submitted June 21, 2002
Returned for revision July 8, 2002
Accepted August 5, 2002

Reproductive Technology


Effect of pH Value of Freeze-Drying Solution on the Chromosome Integrity and Developmental Ability of Mouse Spermatozoa

Takehito Kaneko 1*, David G. Whittingham 1, Ryuzo Yanagimachi 1
1 Institute for Biogenesis Research

* To whom correspondence should be addressed. E-mail: takehito{at}hawaii.edu.

Abstract

The nuclei of freeze-dried mouse spermatozoa are able to retain their chromosome integrity and developmental potential. To optimize the conditions of freeze-drying we examined whether pH values of the freeze-drying solution affect the chromosome integrity and developmental potential of sperm nuclei. The sperm freeze-drying solution we used contained a high concentration (50 mM) of calcium-chelating EGTA. Sperm chromosomes were examined at the metaphase of the first mitosis after injection of freeze-dried spermatozoa into matured oocytes. The developmental potential of sperm nuclei was assessed by examining the development of fetuses in mid-gestation. The results showed that both sperm chromosomes and sperm developmental potential are maintained better when the freeze-drying solution was slightly alkaline (pH 8.0) rather than near neutral or acidic (pH 7.4-6.0). The data indicated that the chromosome integrity and developmental ability of mouse spermatozoa are affected by the pH value of freeze- drying solution.



Key words: Embryo • Fertilization • Sperm


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