Biol Reprod Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

BOR - Papers in Press, published online ahead of print February 19, 2003.
Biol Reprod 2003, 10.1095/biolreprod.102.012609
This Article
Right arrow Full Text (Rapid PDF)
Right arrow All Versions of this Article:
69/1/37    most recent
biolreprod.102.012609v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yu, Z.
Right arrow Articles by Han, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yu, Z.
Right arrow Articles by Han, D.
Agricola
Right arrow Articles by Yu, Z.
Right arrow Articles by Han, D.
Submitted November 1, 2002
Returned for revision December 3, 2002
Accepted February 3, 2003

Gamete Biology


Gene Expression Profiles in Different Stages of Mouse Spermatogenic Cells During Spermatogenesis

Zuoren Yu , Rui Guo , Yehua Ge , Jing Ma , Jikui Guan , Sai Li , Xiaodong Sun , Shepu Xue , Daishu Han *

* To whom correspondence should be addressed. E-mail: daishu{at}public.bta.net.cn.

Abstract

During spermatogenesis, diploid stem cells differentiate, undergo meiosis and spermiogenesis, and transform into haploid spermatozoa. Various factors have been demonstrated to regulate this marvelous process of differentiation, but the expression of only a few genes specifically involved in spermatogenesis has been studied. In the present study, different types of spermatogenic cells were isolated from different ages of Balb/c mice testes using velocity sedimentation method, and we determined the expression profiles of 1176 known mouse genes in 6 different types of mouse spermatogenic cells (primitive type A spermatogonia, type B spermatogonia, preleptotene spermatocytes, pachytene spermatocytes, round spermatids and elongating spermatids) using atlas cDNA arrays. Of the 1176 genes on the Atlas Mouse 1.2 Expression Arrays, we detected 181 genes in primitive type A spermatogonia, 256 in type B spermatogonia, 221 in preletotene spermatocytes, 160 in pachytene spermatocytes, 141 in round spermatids and 126 in elongating spermatids. A number of genes were detected as differential expression (up-regulation or down-regulation). Fourteen of the differentially expressed genes have been further confirmed by RT-PCR for their expression characterizations in different types of spermatogenic cells. These results provide more information for further studies into spermatogenesis-related genes and may lead to the identification of genes with potential relevance to spermatogenesis.



Key words: Testis • Gametogenesis • Sperm • Spermatogenesis


This article has been cited by other articles:


Home page
 ReproductionHome page
N. Yan, Y. Lu, H. Sun, D. Tao, S. Zhang, W. Liu, and Y. Ma
A microarray for microRNA profiling in mouse testis tissues
Reproduction, July 1, 2007; 134(1): 73 - 79.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
B. de Montgolfier, J. Dufresne, M. Letourneau, J. J. Nagler, A. Fournier, C. Audet, and D. G. Cyr
The Expression of Multiple Connexins Throughout Spermatogenesis in the Rainbow Trout Testis Suggests a Role for Complex Intercellular Communication
Biol Reprod, January 1, 2007; 76(1): 2 - 8.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
S. Fouchecourt, M. Godet, O. Sabido, and P. Durand
Glial cell-line-derived neurotropic factor and its receptors are expressed by germinal and somatic cells of the rat testis.
J. Endocrinol., July 1, 2006; 190(1): 59 - 71.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
A Wagener, J Fickel, J Schon, A Fritzenkotter, F Goritz, and S Blottner
Seasonal variation in expression and localization of testicular transforming growth factors TGF-{beta}1 and TGF-{beta}3 corresponds with spermatogenic activity in roe deer
J. Endocrinol., November 1, 2005; 187(2): 205 - 215.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
K Wagoner, G Sanchez, A-N Nguyen, G C Enders, and G Blanco
Different expression and activity of the {alpha}1 and {alpha}4 isoforms of the Na,K-ATPase during rat male germ cell ontogeny
Reproduction, November 1, 2005; 130(5): 627 - 641.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
Z. Yu, T. Raabe, and N. B. Hecht
MicroRNA Mirn122a Reduces Expression of the Posttranscriptionally Regulated Germ Cell Transition Protein 2 (Tnp2) Messenger RNA (mRNA) by mRNA Cleavage
Biol Reprod, September 1, 2005; 73(3): 427 - 433.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
N. H. Ing, A. M. Laughlin, D. D. Varner, T. H. Welsh Jr., D. W. Forrest, T. L. Blanchard, and L. Johnson
Gene Expression in the Spermatogenically Inactive "Dark" and Maturing "Light" Testicular Tissues of the Prepubertal Colt
J Androl, July 1, 2004; 25(4): 535 - 544.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
K. Almstrup, J. E. Nielsen, M. A. Hansen, M. Tanaka, N. E. Skakkebaek, and H. Leffers
Analysis of Cell-Type-Specific Gene Expression During Mouse Spermatogenesis
Biol Reprod, June 1, 2004; 70(6): 1751 - 1761.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
P.J.I. Ellis, R.A. Furlong, A. Wilson, S. Morris, D. Carter, G. Oliver, C. Print, P.S. Burgoyne, K.L. Loveland, and N.A. Affara
Modulation of the mouse testis transcriptome during postnatal development and in selected models of male infertility
Mol. Hum. Reprod., April 1, 2004; 10(4): 271 - 281.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
K.-F. Lee, W. S.B. Yeung, J. F.C. Chow, C. K. Shum, and J. M. Luk
Different Testicular Gene Expression Patterns in the First Spermatogenic Cycle of Postnatal and Vitamin A-Deficient Rat Testis
Biol Reprod, April 1, 2004; 70(4): 1010 - 1017.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
A. L. Y. Pang, H. C. Taylor, W. Johnson, S. Alexander, Y. Chen, Y. A. Su, X. Li, N. Ravindranath, M. Dym, O. M. Rennert, et al.
Identification of Differentially Expressed Genes in Mouse Spermatogenesis
J Androl, November 1, 2003; 24(6): 899 - 911.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2003 by the Society for the Study of Reproduction.