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BOR - Papers in Press, published online ahead of print August 6, 2003.
Biol Reprod 2003, 10.1095/biolreprod.103.017384
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Submitted March 20, 2003
Returned for revision April 6, 2003
Accepted August 4, 2003

Embryo


Identification of a Biological Activity that Supports Maintenance and Proliferation of Pluripotent Cells from the Primitive Ectoderm of the Mouse

Joy Rathjen , Jennifer M. Washington , Michael D. Bettess , and Peter D. Rathjen *

* To whom correspondence should be addressed. E-mail: peter.rathjen{at}adelaide.edu.au.

Abstract
Pluripotent cell development in the mammalian embryo results in the sequential formation of several, developmentally distinct populations, inner cell mass, primitive ectoderm and the primordial germ lineage. Factors within medium conditioned by HepG2 cells (MEDII) have been implicated in the formation and maintenance of primitive ectoderm from inner cell mass cells both in vitro and in vivo. Here we demonstrate that MEDII, but not LIF, is able to support the maintenance and proliferation in culture of pluripotent cells derived from primitive ectoderm formed in vitro or during embryonic development. This distinguishes primitive ectoderm and ICM on the basis of cytokine responsiveness, and validates the biological activity proposed for factors within MEDII in primitive ectoderm establishment and maintenance. Further, it potentially provides an alternative technology for the isolation of pluripotent cells from the mammalian embryo.

Key words: Developmental biology • Early development • Gene regulation • Growth factors


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A. M. Wobus and K. R. Boheler
Embryonic Stem Cells: Prospects for Developmental Biology and Cell Therapy
Physiol Rev, April 1, 2005; 85(2): 635 - 678.
[Abstract] [Full Text] [PDF]


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