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Abstract
Mouse spermatozoa from the caudae epididymides could be
freeze-dried without losing their ability to support
normal development. Immature spermatozoa from the testes,
in contrast, were damaged by freeze-drying. However,
immature spermatozoa became resistant to freeze-drying
after their treatment with diamide, which oxidizes free
-SH groups. Conversely, epididymal spermatozoa were
damaged by freeze-drying if first treated with
dithiothreitol (DTT), which reduces -SS- bonds. The
potential for freeze-drying damage seems likely to relate
to the -SS- status of sperm proteins, in particular its
protamines.
Key words:
Gamete Biology •
Testis •
Epididymis •
Fertilization •
Sperm
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