Biol Reprod Lalor Postdoctoral Fellowships -- Application Deadline January 15, 2009
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

BOR - Papers in Press, published online ahead of print June 11, 2003.
Biol Reprod 2003, 10.1095/biolreprod.103.019950
This Article
Right arrow Full Text (Rapid PDF)
Right arrow All Versions of this Article:
69/5/1525    most recent
biolreprod.103.019950v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Enright, B. P.
Right arrow Articles by Tian, X. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Enright, B. P.
Right arrow Articles by Tian, X. C.
Agricola
Right arrow Articles by Enright, B. P.
Right arrow Articles by Tian, X. C.
Submitted June 3, 2003
Returned for revision June 5, 2003
Accepted June 5, 2003

Reproductive Technology


Epigenetic Characteristics of Bovine Donor Cells for Nuclear Transfer: Levels of Histone Acetylation

B. P. Enright , B. S. Jeong , X. Yang , and X. C. Tian *

* To whom correspondence should be addressed. E-mail: xtian{at}canr.uconn.edu.

Abstract

Donor cell type, cell cycle stage, and passage number of cultured cells all affect the developmental potential of cloned embryos. Since acetylation of the histones on nuclear chromatin is an important aspect of gene activation, this study investigated the differences in histone acetylation of bovine fibroblast and cumulus cells at various passages and cell cycle stages. The acetylation was qualitatively analyzed by epi-flourescent confocal microscopy and quantitatively by immuno-fluorescent flow cytometry. Specifically, we studied levels of histone H4 acetylated at lysine 8, and histone H3 acetylated at lysine 18. Acetylation at these lysine residues is among the most common for the above histone molecules. We also studied levels of linker histone H1 in donor cells. Our results show that stage of cell cycle, cell type, and numbers of cell passages all had an effect on histone content. Histone H1 and acetyl histone H3 increased with cell passage (passage 5 to 15) in G0/G1 and G2/M stage cumulus and fibroblast cells. We also found that acetyl histone H4 was lower in early versus late cell passages (P5 vs. P15) for G0/G1 stage cumulus cells. In both cell types examined, acetyl histones increased with cell cycle progression from G0/G1 into the S and G2/M phases. These results indicate that histone acetylation status is re-modeled by in vitro cell culture and this may have implications for nuclear transfer.



Key words: Assisted Reproductive Technology • Cumulus cells


This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
A. M. Giraldo, D. A. Hylan, C. B. Ballard, M. N. Purpera, T. D. Vaught, J. W. Lynn, R. A. Godke, and K. R. Bondioli
Effect of Epigenetic Modifications of Donor Somatic Cells on the Subsequent Chromatin Remodeling of Cloned Bovine Embryos
Biol Reprod, May 1, 2008; 78(5): 832 - 840.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
L. H. Shi, J. S. Ai, Y. C. OuYang, J. C. Huang, Z. L. Lei, Q. Wang, S. Yin, Z. M. Han, Q. Y. Sun, and D. Y. Chen
Trichostatin A and nuclear reprogramming of cloned rabbit embryos
J Anim Sci, May 1, 2008; 86(5): 1106 - 1113.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
F. Yang, R. Hao, B. Kessler, G. Brem, E. Wolf, and V. Zakhartchenko
Rabbit somatic cell cloning: effects of donor cell type, histone acetylation status and chimeric embryo complementation
Reproduction, January 1, 2007; 133(1): 219 - 230.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
T. Suteevun, R. Parnpai, S. L. Smith, C-C. Chang, S. Muenthaisong, and X. C. Tian
Epigenetic characteristics of cloned and in vitro-fertilized swamp buffalo (Bubalus bubalis) embryos
J Anim Sci, August 1, 2006; 84(8): 2065 - 2071.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
B.P. Enright, L.-Y. Sung, C.-C. Chang, X. Yang, and X.C. Tian
Methylation and Acetylation Characteristics of Cloned Bovine Embryos from Donor Cells Treated with 5-aza-2'-Deoxycytidine
Biol Reprod, April 1, 2005; 72(4): 944 - 948.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
S. Li, Y. Li, W. Du, L. Zhang, S. Yu, Y. Dai, C. Zhao, and N. Li
Aberrant Gene Expression in Organs of Bovine Clones That Die Within Two Days after Birth
Biol Reprod, February 1, 2005; 72(2): 258 - 265.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2003 by the Society for the Study of Reproduction.