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BOR - Papers in Press, published online ahead of print December 17, 2003.
Biol Reprod 2003, 10.1095/biolreprod.103.020198
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Submitted June 11, 2003
Returned for revision July 7, 2003
Accepted December 1, 2003

Pregnancy


Expression of Trophoblast Cell-Specific Pregnancy-Related Genes in Somatic-Cell-Cloned Bovine Pregnancies

Osman V. Patel , Osamu Yamada , Keiichiro Kizaki , Toru Takahashi , Kei Imai , Seiya Takahashi , Yoshiaki Izaike , Linda A. Schuler , Toshiaki Takezawa , and Kazuyoshi Hashizume *

* To whom correspondence should be addressed. E-mail: kazuha{at}iwate-u.ac.jp.

Abstract
We compared the expression of bovine prolactin-related protein-1 (bPRP-1), placental lactogen (bPL), pregnancy-associated glycoproteins-1 (bPAG-1) and -9 (bPAG-9) genes in artificially inseminated (AI) and nuclear-transferred (NT) cows during the first trimester of gestation using real-time reverse transcription PCR (RT-PCR) and in situ hybridization. Placentomal (cotyledonary, caruncular) and interplacentomal (intercotyledonary, intercaruncular) tissues of AI and NT cows carrying either motile (M) or immotile (IM) fetuses were examined. Transcripts for bPL and bPAG-9 were lower (P<0.01) in the fetal membranes of NT (n=4) cows at Day 30 of gestation compared to AI (n=4) cows. There was no difference in the mean(±SEM) levels of expressions of bPRP-1, bPL, and PAG-1 in the placentomal and interplacentomal tissues of AI (n=5) and NT (M, n=4) cows at Day 60 of gestation. Messenger RNAs for bPRP-1, bPL, and bPAGs-1 and -9 genes were higher (P<0.01) in the caruncular tissue of AI cows compared to NT (IM, n=4) cows at Day 60 of gestation. Expression of bPRP-1, bPL, and bPAGs-1 and -9 in the placentomal and interplacentomal tissues of the NT (n=3) group varied considerably more compared to the AI (n=4) group at Day 100 of gestation. These findings suggest defective binucleate cell-specific gene transcriptional commands in NT cows.

Key words: Pregnancy • Conceptus • Placenta


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