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BOR - Papers in Press, published online ahead of print January 14, 2004.
Biol Reprod 2004, 10.1095/biolreprod.103.020461
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Submitted June 18, 2003
Returned for revision July 9, 2003
Accepted December 16, 2003

Ovary


Acquisition of Luteolytic Capacity: Changes in Prostaglandin F2{alpha} Regulation of Steroid Hormone Receptors and Estradiol Biosynthesis in Pig Corpora Lutea

Francisco J. Diaz and Milo C. Wiltbank *

* To whom correspondence should be addressed. E-mail: wiltbank{at}calshp.cals.wisc.edu.

Abstract
The pig CL acquires luteolytic capacity at about day 13 of the estrous cycle after which luteolysis occurs in response to PGF2{alpha} treatment. We postulated that differences in transcription factors such as the steroid hormone receptors may be responsible for the differences in PGF2{alpha}-induced gene expression after acquisition of luteolytic capacity. In these studies we evaluated the effect of PGF2{alpha} on luteal expression of receptors for progesterone (nuclear and membrane PR), estradiol (ER-{alpha} and ER-{beta}), glucocorticoid, and androgens and two enzymes in estradiol synthesis (P450-17{alpha} and aromatase). Two experiments were conducted to examine the early (0.5 hour, experiment I) and late (10 hours, experiment II) effects of PGF2{alpha} on the expression of these receptors in CL with (day 17) or without (day 9) luteolytic capacity. PGF2{alpha} decreased ER-{alpha} mRNA (35%) and increased ER-{beta} mRNA (558 %) and protein (376 %) only in day 17 CL and not day 9. The estradiol biosynthetic pathway was upregulated by PGF2{alpha} in day 17 CL but not day 9 CL with a dramatic increase in aromatase mRNA and luteal estradiol content. Nuclear PR was not affected by PGF2{alpha}, but was greater (176 %) in day 9 CL, while a putative membrane PR was greater (156 %) in day 17, than day 9 CL. There were no detectable changes in glucocorticoid or androgen receptor mRNA. Thus, luteolytic capacity is associated with up-regulation of estradiol biosynthesis, which in conjunction with increased ER-{beta} expression and altered PR expression may promote luteolysis in the pig CL.

Key words: Corpus luteum • Estradiol • Steroid hormone receptors


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