Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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BOR - Papers in Press, published online ahead of print December 10, 2003.
Biol Reprod 2003, 10.1095/biolreprod.103.022079
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Submitted August 7, 2003
Returned for revision September 2, 2003
Accepted December 1, 2003

Male Reproductive Tract


Expression and Regulation of Lipocalin-Type Prostaglandin D Synthase in Rat Testis and Epididymis

Hui Zhu , Hong Ma , Hua Ni , Xing-Hong Ma , Nathaniel Mills , and Zeng-Ming Yang *

* To whom correspondence should be addressed. E-mail: zmyang{at}mail.neau.edu.cn.

Abstract
Lipocalin-type prostaglandin D synthase (L-PGDS), a bi-functional protein, is expressed in the male reproductive organs of many species. However, the expression and regulation of L-PGDS in rat are still uncertain. The present study investigates the regionalization and regulation of L-PGDS expression in rat testis and epididymis by in situ hybridization and immunohistochemistry under the conditions of sexual maturation, castration, and ethylene dimethane sulfonate (EDS) treatments. In sexually mature rats, L-PGDS mRNA was weakly expressed only in the testicular peritubular cells, while L-PGDS immunostaining was highly detected in the Leydig cells by day 70 postpartum. During sexual maturation, L-PGDS mRNA expression was highly detected in the caput, corpus and cauda of the epididymis 70 days after birth. Compared to normal L-PGDS expression in adult epididymis, both L-PGDS mRNA expression and protein immunostaining were significantly reduced in the caput, corpus, and cauda epididymis after castration. Testosterone propionate treatment induced a significant increase of L-PGDS expression in the epididymis of castrated rats. Compared to adult rat epididymis, L-PGDS mRNA and protein expression was down-regulated after EDS treatment. testosterone propionate treatment could induce an increase of L-PGDS mRNA and protein expression in the epididymis of EDS-treated rats. In conclusion, both castration and EDS treatments caused a significant decrease of L-PGDS expression in the epididymis, while testosterone propionate treatment could induce an increase of L-PGDS expression in the epididymis of both castrated and EDS-treated rats, indicating that L-PGDS expression in the rat epididymis can be up-regulated by testosterone.

Key words: Male Reproductive Tract • Testis • Epididymis • Testosterone


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