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Abstract
Successful in vitro maturation (IVM) of oocytes obtained
from medium sized antral follicles could avoid the need
for superovulation for in vitro fertilisation. The wide
range of doses of FSH used in IVM prompted us to study the
effect of varying concentrations of FSH on the dynamics of
nutrient uptake and production by individual maturing
mouse cumulus-oocyte-complexes (COCs). COCs isolated from
the antral follicles of unprimed, prepubertal
B6CBF1 mice were cultured individually in
increasing concentrations of FSH (0 to 2000 ng
ml-1). Following culture, pyruvate,
glucose and lactate uptake or production by individual
complexes were non-invasively assessed and compared to the
stage of nuclear maturation of the enclosed oocyte. FSH
significantly increased oocyte maturation and produced a
2-3 fold increase in glucose uptake and lactate production
by COCs in which the enclosed oocyte completed maturation.
In these COCs, pyruvate was taken up under control
conditions but was produced progressively more in
increasing concentrations of FSH. In COCs where the oocyte
failed to complete maturation, pyruvate was taken up
(rather than produced) and glucose uptake and lactate
production were lower and unaffected by the presence or
absence of FSH. This suggests that there is dialogue
between cumulus cells and the maturing oocyte that
influences FSH responsiveness and substrate metabolism of
the whole COC. Finally, inhibition of FSH-stimulated
glucose uptake by the PI3-kinase inhibitor LY294002, and
the finding of GLUT4 protein in granulosa cells, suggests
that FSH increases glucose uptake by PI3-kinase mediated
translocation of GLUT4 to the granulosa cell membrane.
Key words:
Gamete Biology •
Cumulus cells •
Follicle-stimulating hormone •
Meiosis •
Oocyte development
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