Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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BOR - Papers in Press, published online ahead of print January 28, 2004.
Biol Reprod 2004, 10.1095/biolreprod.103.025510
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Submitted November 13, 2003
Returned for revision November 26, 2003
Accepted January 19, 2004

Ovary


Cell-Specific Expression and Regulation of Soluble Guanylyl Cyclase {alpha}1 and {beta}1 Subunits in the Rat Ovary

Fangxiong Shi , Robert L. Stewart, Jr. , Emerson Perez , Jean Y-H Chen , and Philip S. LaPolt *

* To whom correspondence should be addressed. E-mail: plapolt{at}exchange.calstatela.edu.

Abstract
Soluble guanylyl cyclase (sGC) is activated by nitric oxide (NO) and carbon monoxide (CO), resulting in cGMP production. Recent studies indicate that NO and cGMP influence ovarian functions. However, little information is available regarding the ovarian expression of sGC. This study examined sGC {alpha}1 and {beta}1 subunit protein levels in the ovary during postnatal development, gonadotropin-induced follicular growth, ovulation, and luteinization, and in cultured rat granulosa cells. In postnatal rats, sGC {alpha}1 subunit immunoreactivity was high in granulosa cells of primordial and primary follicles on Day 5, but was low in granulosa cells of larger follicles on Days 10 and 19. Theca cells of developing follicles, but not stromal cells, also demonstrated moderate sGC {alpha}1 immunoreactivity. In gonadotropin-treated immature rats, intense sGC {alpha}1 subunit staining was similarly observed in granulosa cells of primordial and primary follicles, but was low in granulosa cells of small antral follicles and undetectable in granulosa cells of large antral and preovulatory follicles. Following ovulation, corpora lutea expressed moderate sGC {alpha}1 immunoreactivity. Similar ovarian localization and expression patterns were seen for sGC {beta}1, indicting regulated coexpression of sGC subunits. Immunoblot analysis revealed no change in total ovarian sGC {alpha}1 and {beta}1 subunit protein levels during gonadotropin treatment. Similarly, there was no apparent effect of FSH on sGC subunit protein levels in cultured granulosa cells. These findings indicate regulated, cell-specific patterns of sGC expression in the ovary, and are consistent with roles of cGMP in modulating ovarian functions.

Key words: Ovary • Cyclic guanosine monophosphate • Follicular development • Granulosa cells • Nitric oxide


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