Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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BOR - Papers in Press, published online ahead of print February 11, 2004.
Biol Reprod 2004, 10.1095/biolreprod.103.027177
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Submitted December 31, 2003
Returned for revision January 9, 2004
Accepted January 30, 2004

Male Reproductive Tract


Adenosine Triphosphate Concentration and {beta}-D-Glucuronidase Activity as Indicators of Sea Bass Semen Quality

Loredana Zilli , Roberta Schiavone , Vincenzo Zonno , Carlo Storelli , and Sebastiano Vilella *

* To whom correspondence should be addressed. E-mail: sebastiano.vilella{at}unile.it.

Abstract
In this study chemical and biochemical parameters of sea bass sperm were investigated to find out an alternative (to fertilization and motility) method to evaluate sperm fertilization ability before and after cryopreservation procedure. The biochemical and chemical determinations were performed both in fresh and frozen-thawed sperm and seminal plasma. To cryopreserve sperm 250 µl straws were used. Fertilization ability was evaluated by inseminating eggs (obtained from hormonal stimulated females) with fresh and cryopreserved sperm. The results demonstrate the existence of a linear relationship (P<0.05) between semen fertilization capacity and some seminal plasma ({beta}-D-glucuronidase, K+ concentration) and sperm (ATP, aspartate aminotransferase activity) parameters. Variations in semen fertilization rate could be best described by two multiple regression models: firstly, a model including the sperm parameters; secondly, a model including the seminal plasma parameters. For practical application, the use of simple regression models is of value. Fertilization rate both in fresh and cryopreserved sperm is well predicted by determining or the ATP concentration, or the {beta}-D-glucuronidase activity, or both.

Key words: Fertilization • Sperm


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L. Zilli, R. Schiavone, V. Zonno, R. Rossano, C. Storelli, and S. Vilella
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Biol Reprod, May 1, 2005; 72(5): 1262 - 1267.
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