Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

BOR - Papers in Press, published online ahead of print June 16, 2004.
Biol Reprod 2004, 10.1095/biolreprod.104.028621
This Article
Right arrow Full Text (Rapid PDF)
Right arrow All Versions of this Article:
71/4/1296    most recent
biolreprod.104.028621v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zaga, V.
Right arrow Articles by Vadillo-Ortega, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zaga, V.
Right arrow Articles by Vadillo-Ortega, F.
Agricola
Right arrow Articles by Zaga, V.
Right arrow Articles by Vadillo-Ortega, F.
Submitted February 17, 2004
Returned for revision March 5, 2004
Accepted June 7, 2004

Pregnancy


Secretions of Interleukin-1 {beta} and Tumor Necrosis Factor {alpha} by Whole Fetal Membranes Depend on Initial Interactions of Amnion or Choriodecidua with Lipopolysaccharides or Group B Streptococci

Veronica Zaga , Guadalupe Estrada-Gutierrez , Jorge Beltran-Montoya , Rolando Maida-Claros , Rosario Lopez-Vancell , and Felipe Vadillo-Ortega *

* To whom correspondence should be addressed. E-mail: felipe.vadillo{at}uia.mx.

Abstract
This study evaluated the secretions of interleukin-1 beta (IL-1{beta}) and tumor necrosis factor alpha (TNF-{alpha}) by fetal membranes stimulated with group B Streptococcus (GBS) and lipopolysaccharide (LPS). Aims of the study were directed to evaluate the initial response of full thickness membranes to the microbial insult, using an in vitro experimental model allowed testing of the individual contribution of amnion and choriodecidua to stimulation. Full thickness membranes were obtained after delivery by elective cesarean section from women at 37-40 weeks of gestation without evidence of active labor. The membranes were mounted in Transwell devices, physically separating the upper and lower chambers. LPS (500 ng/mL) or GBS (1 x 10 6 CFU) was added to either amniotic or choriodecidua surface and accumulation of IL-1{beta} and TNF-{alpha} were measured in both compartments using specific ELISA. Fetal membranes followed different patterns of secretion of proinflammatory cytokines that depended on the side to which the stimulus was added or the nature of the stimulus itself. TNF-{alpha} was secreted by amnion and choriodecidua in the presence of LPS or GBS, and stimulation with GBS induced a greater synthesis of IL-1 {beta} than did LPS. Choriodecidua tissue was more responsive than amniotic tissue, and this response tended to be higher even when the stimulation was only on the amniotic side. However, the amnion plays an active role in recognizing LPS or GBS, contributing a significant amount of TNF-{alpha}. Thus, there are cooperative and bidirectional communications between amnion and choriodecidua in response to bacterial products, which include intermembranous cytokine traffic and signaling between tissues.

Key words: Pregnancy • Cytokines • Parturition • Placenta


This article has been cited by other articles:


Home page
 J. Clin. Endocrinol. Metab.Home page
C. Oner, F. Schatz, G. Kizilay, W. Murk, L. F. Buchwalder, U. A. Kayisli, A. Arici, and C. J. Lockwood
Progestin-Inflammatory Cytokine Interactions Affect Matrix Metalloproteinase-1 and -3 Expression in Term Decidual Cells: Implications for Treatment of Chorioamnionitis-Induced Preterm Delivery
J. Clin. Endocrinol. Metab., January 1, 2008; 93(1): 252 - 259.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
G. Chevillard, A. Derjuga, D. Devost, H. H Zingg, and V. Blank
Identification of interleukin-1{beta} regulated genes in uterine smooth muscle cells
Reproduction, December 1, 2007; 134(6): 811 - 822.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
G. Garcia-Lopez, F. Vadillo-Ortega, H. Merchant-Larios, R. Maida-Claros, M. Osorio, D. Soriano-Becerril, H. Flores-Herrera, J. Beltran-Montoya, Y. Garfias-Becerra, and V. Zaga-Clavellina
Evidence of in vitro differential secretion of 72 and 92 kDa type IV collagenases after selective exposure to lipopolysaccharide in human fetal membranes
Mol. Hum. Reprod., June 1, 2007; 13(6): 409 - 418.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
M.-J. Leroy, E. Dallot, I. Czerkiewicz, T. Schmitz, and M. Breuiller-Fouche
Inflammation of Choriodecidua Induces Tumor Necrosis Factor Alpha-Mediated Apoptosis of Human Myometrial Cells
Biol Reprod, May 1, 2007; 76(5): 769 - 776.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
C. J. Lockwood, F. Arcuri, P. Toti, C. D. Felice, G. Krikun, S. Guller, L. F. Buchwalder, and F. Schatz
Tumor Necrosis Factor-{alpha} and Interleukin-1{beta} Regulate Interleukin-8 Expression in Third Trimester Decidual Cells: Implications for the Genesis of Chorioamnionitis
Am. J. Pathol., October 1, 2006; 169(4): 1294 - 1302.
[Abstract] [Full Text] [PDF]

Home page
 Reproductive SciencesHome page
V. Zaga-Clavellina, H. Merchant-Larios, G. Garcia-Lopez, R. Maida-Claros, and F. Vadillo-Oretega
Differential Secretion of Matrix Metalloproteinase-2 and -9 After Selective Infection With Group B Streptococci in Human Fetal Membranes
Reproductive Sciences, May 1, 2006; 13(4): 271 - 279.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2004 by the Society for the Study of Reproduction.