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BOR - Papers in Press, published online ahead of print November 24, 2004.
Biol Reprod 2004, 10.1095/biolreprod.104.029058
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Submitted March 1, 2004
Returned for revision March 16, 2004
Accepted November 2, 2004

Pregnancy


Variation in Macrophage-Migration-Inhibitory-Factor Immunoreactivity During Porcine Gestation

Luana Paulesu , Chiara Cateni , Roberta Romagnoli , Francesca Ietta , and Vibeke Dantzer *

* To whom correspondence should be addressed. E-mail: vibeke.dantzer{at}iaf.kvl.dk.

Abstract
The localization and activity of Macrophage Migration Inhibitory Factor (MIF) was investigated in the interhemal region of the non-invasive, diffuse, folded epitheliochorial placenta and in the non pregnant uterus of the pig. MIF, a proinflammatory cytokine with many actions on macrophages and monocytes, may play an important role in the materno-fetal immunotollerance during placental establishment, modulation and growth. Immunohistochemical staining with anti-human MIF polyclonal antibodies was carried out on placental sections from eleven stages of gestation (16-95 days post coitus) and on non-pregnant uterus at 13 days post estrus. Western blot analysis confirmed the specificity of the anti- human MIF polyclonal antibodies on pig tissues. MIF staining was intense in both the trophoblast and maternal epithelium in the early stages; in the later stages, it decreased dramatically in the maternal epithelium but remained high in the trophoblast. The uterine glands showed immunoreactivity at all stages and the maternal and fetal epithelial lining of the areolar cavity showed high reactivity at day 25. The vasculature also showed staining for MIF and an intense to moderate staining was shown in the non-pregnant uterus mostly in the surface and glandular epithelium. The high activity of MIF in the maternal and fetal tissues throughout placentation and its expression in the non-pregnant uterus indicate a regulatory role of MIF during embryo receptivity and epitheliochorial placentation.

Key words: Immunology • Pregnancy • Cytokines • Placenta • Trophoblast


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[Abstract] [PDF]


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