Biol Reprod Lalor Postdoctoral Fellowships -- Application Deadline January 15, 2009
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

BOR - Papers in Press, published online ahead of print June 30, 2004.
Biol Reprod 2004, 10.1095/biolreprod.104.029322
This Article
Right arrow Full Text (Rapid PDF)
Right arrow All Versions of this Article:
71/5/1578    most recent
biolreprod.104.029322v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kurosaka, S.
Right arrow Articles by McLaughlin, K. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kurosaka, S.
Right arrow Articles by McLaughlin, K. J.
Agricola
Right arrow Articles by Kurosaka, S.
Right arrow Articles by McLaughlin, K. J.
Submitted March 5, 2004
Returned for revision April 2, 2004
Accepted June 25, 2004

Embryo


Pluripotent Lineage Definition in Bovine Embryos by Oct4 Transcript Localization

Satoshi Kurosaka , Sigrid Eckardt , and K. John McLaughlin *

* To whom correspondence should be addressed. E-mail: kjmclaug{at}vet.upenn.edu.

Abstract
The POU-domain transcription factor Pou5f1 (Oct4) is restricted to pluripotent embryonic cells and the germ line of the mouse and is required for maintenance of pluripotency of cells within the inner cell mass of the mouse blastocyst. Despite highly conserved genomic organization and regulatory regions between the mouse Oct4 gene and its bovine ortholog, bovine Oct4 protein is not restricted to the inner cell mass of blastocyst stage embryos, suggesting that Oct4 may not be a key regulator of pluripotency in the bovine. We have analyzed the temporal and spatial distribution of Oct4 transcript in bovine oocytes and preimplantation stage embryos, and, in contrast to protein distribution, find strong conservation between bovine and mouse. Oct4 transcript is present at low levels in the bovine oocyte. Similar to mouse, bovine Oct4 transcription begins one to two cell cycles after zygotic genome activation followed by a sharp increase in transcription subsequent to compaction. Oct4 transcript is ubiquitously present in all cells of embryos at the morula stage, however, in Day 7 bovine blastocysts, Oct4 signal is not visible in the trophectoderm by in situ hybridization, indicating that transcriptional downregulation of Oct4 upon differentiation is similar to that observed in mouse and other mammals. These results indicate that in contrast to protein distribution, regulation of Oct4 transcription is conserved between mammalian species.

Key words: Embryo • Early development • Gene regulation


This article has been cited by other articles:


Home page
 J. Immunol.Home page
Y. Nagai, H. Aso, H. Ogasawara, S. Tanaka, Y. Taketa, K. Watanabe, S. Ohwada, M. T. Rose, H. Kitazawa, and T. Yamaguchi
Anterior Pituitary Progenitor Cells Express Costimulatory Molecule 4Ig-B7-H3
J. Immunol., November 1, 2008; 181(9): 6073 - 6081.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
L. Blomberg, K. Hashizume, and C. Viebahn
Blastocyst elongation, trophoblastic differentiation, and embryonic pattern formation
Reproduction, February 1, 2008; 135(2): 181 - 195.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
A. Wuensch, F. A. Habermann, S. Kurosaka, R. Klose, V. Zakhartchenko, H.-D. Reichenbach, F. Sinowatz, K. J. McLaughlin, and E. Wolf
Quantitative Monitoring of Pluripotency Gene Activation after Somatic Cloning in Cattle
Biol Reprod, June 1, 2007; 76(6): 983 - 991.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
P. M. Misica-Turner, F. C. Oback, M. Eichenlaub, D. N. Wells, and B. Oback
Aggregating Embryonic but Not Somatic Nuclear Transfer Embryos Increases Cloning Efficiency in Cattle
Biol Reprod, February 1, 2007; 76(2): 268 - 278.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
G. Cauffman, H. Van de Velde, I. Liebaers, and A. Van Steirteghem
Oct-4 mRNA and protein expression during human preimplantation development
Mol. Hum. Reprod., March 1, 2005; 11(3): 173 - 181.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2004 by the Society for the Study of Reproduction.